Figure 2
From: Proteins of the origin recognition complex (ORC) and DNA topoisomerases on mammalian chromatin
Orc1p in (Orc1-5) together with a considerable part of topo I and topo II cannot be mobilized by nuclease treatment. (A) Isolated nuclei (50 μg DNA) were treated for 15 min with MN in the amounts indicated. DNA concentrations were determined in the supernatants (not shown) and the pellet. (B) DNA in supernatants and the pellets were deproteinized and analyzed by agarose gel electrophoresis and staining with ethidium bromide. (C) Proteins in the supernatants and the pellets were investigated by western blotting.