Figure 1From: Proteins of the origin recognition complex (ORC) and DNA topoisomerases on mammalian chromatinOrc proteins dissociate together with topoisomerases from chromatin. HeLa cell nuclei were prepared and treated with nuclear extraction (NE) buffer containing 80, 250, 320 and 450 mM NaCl as indicated. The supernatants were analyzed by polyacrylamide gel electrophoresis in the presence of SDS and western blotting.Back to article page