Table 2 Target genes, product sizes and primer sequences used for quantitative real-time PCR
Target genes | Sequences of primer (5'-3') | Product sizes (kB) |
|---|---|---|
CEBP-α (NM_007678.2) | ||
Forward | gctttttgcacctccaccta | 170 |
Reverse | ccacaaagcccagaaaccta | |
CEBP-β (NM_009883.1) | ||
Forward | caagctgagcgacgagtaca | 157 |
Reverse | cagctgctccaccttcttct | |
HNF-1β (NM_009330.2) | ||
Forward | gacactcctcccatcctcaa | 156 |
Reverse | ctccctctgggggatattgt | |
HNF-3α (NM_008259.2) | ||
Forward | cagcacaagctggacttcaa | 173 |
Reverse | agcacgggtctggaatacac | |
HNF-3β (NM_010446.1) | ||
Forward | taagcgagctaaagggagca | 176 |
Reverse | agagaaggggtggttgaagg | |
HNF-4α (NM_008261.2) | ||
Forward | agaggttctgtcccagcaga | 169 |
Reverse | atccagaaggagttcgcaga | |
HNF-6 (NM_008262.2) | ||
Forward | ctgtgaaactcccccaggta | 179 |
Reverse | tgaaactaccgctcacgttg | |
GAPDH (NM_199472.1) | ||
Forward | acccagaagactgtggatgg | 172 |
Reverse | cacattgggggtaggaacac |