Figure 2

Influence of the SAGA, ADA and SLIK complexes on E1A dependent transcriptional activation. The indicated yeast deletion strains isogenic to BY4741 (refer to Additional file 2) were transformed with the pSH1834 LexA responsive reporter plasmid and vectors expressing the LexA DBD, or the LexA DBD fused to the indicated portions of E1A. Extracts were prepared from transformed yeast and assayed for β-galactosidase activity as described previously [29] and detailed in the Methods. Experiments were performed in triplicate with s.d's indicated. The asterisks indicate which complexes are expected to be influenced by the individual gene disruptions, as several of these genes encode factors that are components of more than one complex. For example, Spt3 is a component of the SAGA and SLIK complexes, but not the ADA complex.