Reverse transcription-quantitative polymerase chain reaction: description of a RIN-based algorithm for accurate data normalization

Table 2 Correlation between RIN and relative gene expression for 9 genes in the HCT116, BxPC-3 and A427 cell lines

Gene	HCT116			BxPC-3			A427
	Slope	Intercept	R²	Slope	Intercept	R²	Slope	Intercept	R²
18S	0.09	0.16	0.98	0.14	-0.41	1.00	0.11	-0.16	0.94
ACTB	0.06	0.42	0.92	0.10	-0.03	0.94	0.06	0.41	0.99
ATUB	0.10	-0.01	0.96	0.14	-0.41	0.99	0.10	-0.01	0.95
B2M	0.10	-0.03	0.92	0.10	0.03	0.92	0.07	0.34	0.92
GAPDH	0.08	0.27	0.94	0.10	0.02	0.98	0.08	0.22	0.98
HPRT	0.09	0.08	0.94	0.10	0.05	0.86	0.08	0.16	0.96
POLR2L	0.06	0.4	0.98	0.06	0.43	0.92	0.04	0.60	0.97
PSMB6	0.07	0.35	0.97	0.09	0.14	0.99	0.05	0.53	0.99
RPLP0	0.09	0.09	0.94	0.11	-0.07	0.99	0.06	0.33	0.97

A regression analysis of the relationship between relative gene expression ratios and RIN was performed such that the expression ratio follows the relationship y = a × RIN + b, a being the slope and b the intercept. The coefficients of determination (R²) are also presented.

ISSN: 1471-2199