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Figure 3 | BMC Molecular Biology

Figure 3

From: Reverse transcription-quantitative polymerase chain reaction: description of a RIN-based algorithm for accurate data normalization

Figure 3

Determination of a RIN-based normalizing factor. (A) The measured expression ratios of 9 genes in HCT116, BxPC-3, and A427 samples with decreasing RNA integrity allowed the determination of an average gene degradation profile that follows the equation: relative expression ratio = 0.08 × RIN + 0.19. (B) A RIN-based normalizing factor was determined from the deviation between the average gene degradation and the line y = 1, which corresponds to the expected ratio for intact samples (RIN = 10).

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BMC Molecular Biology

ISSN: 1471-2199

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