Figure 6

RT-PCR analysis of Rv1417 and RibH in M. tuberculosis and M. bovis. Co-transcription of Rv1417 and RibH was studied by RT-PCR assays. Total RNA from M. tuberculosis H37Rv (lanes 1 and 2) and from M. bovis AN5 (lanes 3 and 4) was reverse-transcribed and amplified with the primers LeftOP1417/RightOP1417 (table 1). Lanes 1 and 3, RT-PCR amplification with RT; lanes 2 and 4, RT-PCR amplification without RT; lane 5, M. tuberculosis DNA (positive control); lane 6, PCR negative control; lane 7 M. bovis DNA (positive control). Arrow indicates the size of the bands.