Skip to main content

Table 1 Oligonucleotides used as primers in this study

From: Co-regulated expression of HAND2 and DEIN by a bidirectional promoter with asymmetrical activity in neuroblastoma

method

name

nucleotide sequence

3'-RACE

GSPH1

5'-CATGGACCTGCTGGCCAAGGACG-3'

5'-RACE

GSPH2

5'-CTGAATGAGCCTTGGAGCTCGAAG-3'

 

GSPD1

5'-GCCAATCTGCAGTTTGTCTTTCTG-3'

reporter gene constructs

BPF

5'-TTTTAAGCTT ACATCTTTAGGGCCGCTCG-3'

 

BPR

5'-TTTTAAGCTT GTTAAACACTTGCATTATCCTCTG-3'

 

CDF

5'-TTTTGGATCC ACCTTTCGCCGGAGGCGA-3'

 

CDR

5'-TTTTGGATCC AGCGAGCGGCTGCAGATTTG-3'

 

CHF

5'-TTTTACGCGT ACCTTTCGCCGGAGGCGA-3'

 

CHR

5'-TTTTACGCGT AGCGAGCGGCTGCAGATTTG-3'

 

BDF

5'-TTTTGGATCC GTTTGTAATAAGAGAATGACCGAA-3'

 

BDR

5'-TTTTGGATCC AGGTCTCCTTGGTAATTTGGGTAC-3'

 

BHF

5'-TTTTACGCGT GTTTGTAATAAGAGAATGACCGAA-3'

 

BHR

5'-TTTTACGCGT AGGTCTCCTTGGTAATTTGGGTAC-3'

RT-PCR & Northern Blot

F1

5'-AGCTGTACATGGAGATCTTGC-3'

 

F2

5'-AAAATCAAGACCCTGCGCCTG-3'

 

F3

5'-GCGAAATGAGTCTGGTAGGTG-3'

 

F4

5'-CGACCCATGTAATATGTAACA-3'

 

F5

5'-AATGGGATTCTCTATTTGTGCTG-3'

 

F6

5'-GAAGGCACAGATCATTCATGG-3'

 

R1

5'-CTCACTGTGCTTTTCAAGATTTC-3'

 

R2

5'-CTTGTCGTTGCTGCTCACT-3'

 

R3

5'-CACAGTGGTTTATTGAATACTTAC-3'

 

R4

5'-CACCT ACCAGACTCA TTTCGC-3'

 

R5

5'-TCAGCTAGAAAACTGTATAAGAG-3'

 

R6

5'-ACCACAAGCAGTCTCATGGGA-3'

 

R7

5'-CAGTAAAAAAAACAGTTTGAAAGGC-3'

  1. Forward primers are denoted F, reverse primers are denoted R; GSPH1-GSPD1 are gene specific primers for 3'- and 5'-RACE. BPF-BHR were used for amplification of fragments for subsequent cloning of reporter gene constructs A–F (restriction sites are underlined). F1–6 and R1–7 are primers used for RT-PCR and generation of Northern Blot probes.
Back to article page

BMC Molecular Biology

ISSN: 1471-2199

Contact us