Figure 4

Sequence alignment of the complete nucleotide sequence of the bidirectional promoter between HAND2 and DEIN in human (hs), chimpanzee (pt), mouse (mm), rat (rn), chicken (gg) and zebrafish (dr). First exons of HAND2 and DEIN are shaded in grey. Direction of transcription is indicated by black arrows. Nucleotides are numbered relative to the first nucleotide of the bidirectional promoter. Putative Trancription Factor Binding Sites (TFBS) are boxed. The directionality of TFBS is indicated by arrowheads and color of boxes (<, blue, HAND2-orientation; >, red, DEIN-orientation). SP-1, specificity protein 1; AP-1 and AP-2, activator protein 1 and 2; CdxA, CdxA homeobox gene; Abd-B, Abdominal-B; CREB, cAMP response element-binding protein; Prx2, paired related homeobox 2.