Figure 3
Genomic organization of the human HAND2 - DEIN locus on 4q33–34 and luciferase reporter-gene constructs A-H (A); Relative luciferase activity of constructs A-H in NB cell lines SK-N-AS, SH-EP, SH-SY5Y and Kelly (B). Boxes and lines indicate exons and introns, respectively. Arrows indicate direction of transcription. Blue bars, DEIN orientation; green bars, HAND2 orientation; BP, Bidirectional Promoter; luc, luciferase; BAE, HAND2 Branchial Arch Enhancer; C, putative cis-regulatory element; SVE/SVP, SV40 enhancer- and promoter-elements, respectively. Values of transfection experiments are shown as the mean ± standard deviation of 3 independent experiments. Values are normalized to the activity of the pGL3-Basic vector set as 1.