Figure 5

Expression analysis of Rad51d alternative transcripts. (A) RT-PCR for Mus musculus Rad51d and selected splice variants was conducted in eight tissues. Expression of RAD51DΔ3 employing primers Rad51d F1 and R1 (upper panel); RAD51DΔ5 with Rad51d F2 and R2 (lower panel). (B) RT-PCR products of human RAD51D in normal breast. Arrows indicate transcripts corresponding to known human splice isoforms [16]. RAD51DΔ3 and RAD51DΔ5 transcript positions are underlined. Size markers in base pairs are shown on the left. Numbers in parenthesis represent transcript abundance as determined by ImageJ analysis of the band intensities (bk; background). (C) The RAD51D peptide is aligned with the predicted amino acid sequence of RAD51DΔ9,10 and RAD51DΔ10. RAD51DΔ9,10 contains the first 8 exons of RAD51D followed by four out of frame amino acids (underlined and italicized) encoded by intron 8. RAD51DΔ10 includes amino acids 1–302 of RAD51D followed by five novel amino acids encoded by intronic sequence 5980 bp downstream from the predicted Rad51d polyA site. Premature termination codons are indicated by an asterisk, and exon boundaries are represented by a gap in the alignment of the predicted amino acid sequences. (D) RT-PCR for RAD51DΔ9,10 (upper panel) and RAD51DΔ10 (lower panel) from Mus musculus tissues with primers Rad51d F3 and Rad51dΔ9,10 R1 or Rad51dΔ10 R1 respectively. Abbreviations: 51D; RAD51D-FL, Δ3; RAD51DΔ3, Δ5; RAD51DΔ5, Δ9,10; RAD51DΔ9,10, Δ10; RAD51DΔ10.