Figure 2
From: Functional characterization and identification of mouse Rad51d splice variants
Interaction of the RAD51D alternative isoforms with RAD51C and XRCC2. (A) Sixty-four yeast two-hybrid interactions were tested by plating diploid strains on selective growth medium lacking adenine, leucine, histidine, and tryptophan. (B) Colony lift assays were performed to qualitatively assess β-galactosidase activity in mated yeast. (C) Interactions between isoforms of RAD51D with RAD51C and XRCC2 were quantified by measuring β-galactosidase activity using o-nitrophenyl-β-D-galactopyranoside as a substrate. The binding capacity of the splice variants with RAD51C and XRCC2 are displayed. Experiments were performed in triplicate with error bars representing standard error of the mean. Abbreviations: 51C; RAD51C, 51D; RAD51D-FL, X2; XRCC2, pGAD; pGADT7 vector, pGBK; pGBKT7 vector, AD; activating domain of GAL4, DBD; DNA binding domain of GAL4.