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Table 1 Putative tick RNAi candidate homologues

From: Evidence of a tick RNAi pathway by comparative genomics and reverse genetics screen of targets with known loss-of-function phenotypes in Drosophila

Function

*Protein

R. microplus BmGI2 ID (% Identity)±

I. scapularis contig ID (% Identity)

Ref

DICER (See also Figure 1 & Additional File 1)

RNase III dsRNA processing

*Dcr-1Cele

incomplete

DS643033 (31%)

[12, 13]

Argonaute proteins – target recognition and silencing (See also Figures 2 & 3)

RISC – miRNA pathway

Ago-1

TC13769 (43% DUF1785 and PAZ domains), TC6448 (44% PIWI domain)

ABJB010009424.1 (41%)

[21]

RISC – RNAi pathway

Ago-2

TC8091 (25% DUF1785 & PAZ domains)

ABJB010128003.1 (31%)

[18]

Systemic RNAi (germ cells)

*Rsd-3Cele

MPAAN09TR (45%)

ABJB010279725.1 (48%)

[32]

dsRNA uptake and processing

Endocytic protein (EPsiN)

*Epn-1Cele

BEADR88TR (71%)

ABJB010748067.1 (43%)

[32]

Vesicle mediated transport

AP-50

TC6127 (89%)

ABJB010508398.1 (91%)

[35]

 

Arf72

Not found

ABJB010115816.1 (68%)

[35]

 

Chc (Clathrin hc)

TC10346 (60%

ABJB010065986.1 (87%)

[35]

Endosome transport

Rab7

BEAGW52TR (80%)

ABJB010159881.1 86%)

[35]

Intracellular transport

CG3911

TC6954 (67%)

ABJB010384785 (64%)

[35]

 

Cog3

TC5984 (49%)

ABJB010296208.1 (68%)

[35]

 

ldlCp

Not found

ABJB011123114.1 (52%)

[35]

Lysosomal transport

Lt

TC12854 (35%)

Not found

[35]

Lipid metabolism

Gmer

TC9381 (62%)

Not found

[35]

 

Pi3K59F

BEADT89TR (52%)

Not found

[35]

 

Sap-r

TC9046 (22%)

Not found

[35]

Proteolysis and peptidolysis

CG4572

TC6395 (35%)

ABJB010180836.1 (43%)

[35]

 

CG5053

Not found

ABJB010804049.1 (63%)

[35]

 

CG8184

Not found

ABJB010385401.1 (72%)

[35]

Oogenesis

Egh

TC8075 (67%)

ABJB010259843.1 (66%)

[35]

Rhodopsin mediated signaling

ninaC

Not found

ABJB011087029.1 (42%)

[35]

Translation regulation

Srp72

Not found

ABJB010441811.1 (38%)

[35]

ATP synthase/ATPase

Vha16

MPAA174TR (59%)

ABJB010975295.1 (67%)

[35]

 

VhaSFD

TC10823 (63%)

ABJB010753004 (56%)

[35]

Unknown

CG5161

TC14816 (61%)

No found

[35]

 

CG5382

Not found

ABJB010478954.1 (84%)

[35]

Other factors

    

RISC assembly

Armitage

TC9347 (35%)

Not found

[73]

RISC associated nuclease

TudorSN

BEAFW62TR (46%)

ABJB010481234.1 (48%)

[46]

RISC function

FMRp

BEAE145TR (53%)

ABJB010028120.1 (67%)

[50]

ATP-dependent RNA helicase

Rm62

TC14966 (70%)

ABJB010043214.1 (54%)

[45]

RNA-directed RNA polymerase (see also Figure 4)

*EGO-1Cele

BEAEL55TR (41%)

ABJB010057970.1 (54%)

[28]

  1. *All proteins of the RNAi pathway originate from D. melanogaster, except:Cele = C. elegans; Not found homologues include: C. elegans Rsd-2, Rsd-6, Sid-1, Tag-130 (systemic RNAi), Rde-1, Rde-4 (associated with RNAi machinery); T. casteneum Tc-Sil (systemic RNAi); D. melanogaster Eater, Sr-CI, Sr-CII, Sr-CIII, Sr-CIV (Innate immune response/phagocytosis – dsRNA uptake and processing), CG5434, CG8671 (Unknowns – dsRNA uptake and processing), R2D2, Vasa intronic gene, and Spindle E (associated with RNAi machinery).
  2. ± GenBank accessions for R. microplus tentative consensus sequences and clones are listed in Additional file 6.
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BMC Molecular Biology

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