Figure 6

Developmental expression of Har-POU mRNA detected by semi-quantitative RT-PCR and Southern hybridization. (A) Expression of Har-POU mRNA during pupal development of nondiapause-destined and diapause-destined individuals. Abbreviations: P0–P10, 0–10 days after pupation; RG, rabbit globin. (B) Expression of Har-POU mRNA in diapausing pupae. Abbreviations h and d represent hours and days after injection of ecdysone to diapausing pupa.