Figure 4

RAG-mediated bps6197 cleavage is directed by the inverted repeat sequence and requires the nonamer-like sequence. (A) DNA fragments radiolabeled at the 5'-end were generated by PCR using pGG49 containing or lacking a 23-RSS or their derivatives containing three bp substitutions in the inverted repeat distal to the cleavage site (underlined). (B) The DNA fragments in (A) were subjected to RAG-mediated cleavage as in Figure 2C. (C-D) Radiolabeled DNA fragments were prepared containing a wild-type or mutant (underlined) nonamer-like sequence in bps6197 as described in A and subjected to RAG-mediated cleavage as in (B). Independent experiments reveal similar results.