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Table 1 The 37 bp DNA constructs.

From: A functional type I topoisomerase from Pseudomonas aeruginosa

A.37 bp VacSeq

5'-CGTGTCGATTCCGACGTCCCTTGCATTTATCAATTAT-3'

 

3'-GCACAGCTAAGGCTGCAGGGAACGTAAATAGTTAATA-5'

B.37 bp loops

5'-CCTAGGCTACACCTACTCTTTGTAAGAATTAAGCTTC-3'

 

3'-GGATCCGATGTG CT TGAGAAACA AA CTTAATTCGAAG-5'

C.37 bp no loops

5'-CCTAGGCTACACCTACTCTTTGTAAGAATTAAGCTTC-3'

 

3'-GGATCCGATGTGGATGAGAAACATTCTTAATTCGAAG-5'

  1. (A) The sequence of the 37 bp containing the Vaccinia recognition pentameric sequence in bold.(B) The sequence of the loop-containing duplex: the sequence of the bottom strand is modified to generate tandem mismatches of identical opposing nucleotides with spacing of 9 bp. The sequence generating the loops is in boldface.(C) The sequence of the perfect duplex without loops.
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BMC Molecular Biology

ISSN: 1471-2199

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