Figure 9

Binding of PAT to 37 bp DNA with and without CCCTT-containing sequence. (A) PAT 0, 20, 40, 68, 136, 200, 268, 336, 400, 468, 712 fmol respectively in lanes 1–11 in topoisomerase buffer with MgCl₂. The glycerol concentration in lanes 1 through 11 varies from 8.34% to 24.5%. Free DNA (F), complex (C) and aggregates (A) are indicated on the right.(B) Binding of PAT to 37 bp perfect duplex DNA; PAT 0, 20, 40, 68, 136, 200, 268, 336, 400, 468, 712 fmol respectively in lanes 1–11 in topoisomerase buffer without MgCl₂. The glycerol concentration varies from 8.34% to 24.5% in lanes 1 through 11. Free DNA (F) and aggregates (A) are indicated on the right.