Figure 10

Binding of PAT and Y292F to 37 bp looped DNA. (A) PAT 0, 20, 40, 68, 136, 200, 268, 336, 400, 468, 712 fmol respectively in lanes 1–11 in topoisomerase buffer with MgCl₂ and 37 bp looped DNA. The glycerol concentration in lanes 1 through 11 varies from 8.34% to 24.5%. Free DNA (F), complex (C) and aggregates (A) are indicated on the left. (B) Binding of Y292F to 37 bp looped DNA. Y292F 0, 0.53, 1.06, 1.8, 3.6, 5.3, 7.1, 8.9, 10.6, 12.4, 18.8 pmol respectively in lanes 1–11 in topoisomerase buffer with MgCl₂ and 37 bp looped DNA. The glycerol concentration in lanes 1 through 11 varies from 8.3% to 32.26%. Free DNA (F), complex (C) and aggregates (A) are indicated on the right.