Table 1 nucleotide sequences of primers and the PCR products size
From: Time course analysis of RNA stability in human placenta
TARGET | sequences (primer 3 design) or Qiagen Geneglobe reference number | NCBI reference gene number | NCBI detected transcript number | lenght of amplicon | amplified exons* |
|---|---|---|---|---|---|
GAPDH (fragments) | 4141 | NM_002046 | |||
GAPDH 5'end | |||||
GAPDH 5' Forward | 5'-GAAGGTGAAGGTCGGAGT-3' | 252 bp | 2/3 | ||
GAPDH 5' Reverse | 5'-GAAGATGGTGATGGGATTTC-3' | ||||
GAPDH 3' end | |||||
GAPDH 3' Forward | 5'-AAACCTGCCAAATATGATGACAT-3' | 228 bp | 8/9 | ||
GAPDH 3' Reverse | 5'-ACCCTGTTGCTGTAGCCAAA-3' | ||||
FASN (fragments) | 2194 | NM_004104 | |||
FASN 5' end | |||||
FASN 5' Forward | 5'-ATCCGCTCGTTGTACCAGTC-3' | 281 bp | 7 | ||
FASN 5' Reverse | 5'-GATCTCAGGGTTGGGGCTAT-3' | 9 | |||
FASN 3' end | |||||
FASN 3' Forward | 5'-GGTCTTGAGAGATGGCTTGC-3' | 197 bp | 34/35 | ||
FASN 3' Reverse | 5'-TTGGCAAAGCCGTAGTTG-3' | 36 | |||
House keeping genes | |||||
ALAS1 (Aminolevulinate delta synthase 1) | QT00073122 | 211 | NM_000688 | 113 pb | 9/10 |
B2M (beta2 microglobulin) | QT00088935 | 567 | NM_004048 | 98 pb | 1/2 |
Cyclophilin A (peptidylprolyl isomerase) | |||||
Forward | 5'-TGCTGACTGTGGACAACTCGA-3' | 5478 | NM_203431 | 212 bp | 4 |
Reverse | 5'-TCATAATCATAAACTTAACTCTGCA ATCC-3' | ||||
other genes | |||||
TNFalpha(Tumor necrosis factor) | QT01079561 | 7124 | NM_000594 | 104 bp | 1/2/3 |
COX 2 (cyclooxygenase 2) | QT00040586 | 5743 | NM_000963 | 68 bp | 1/2 |