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Figure 3 | BMC Molecular Biology

Figure 3

From: Time course analysis of RNA stability in human placenta

Figure 3

Analysis of mRNA integrity. Comparison of FASN (a) and GAPDH (b) mRNA 5'/3' ratios in placental samples stored at + 4°C for 0 to 96 h, as determined by qRT-PCR assays targeting sequences close to the 5' and 3' ends of the transcripts. RNA samples prepared with protocol A were compared to protocol B (n = 14 in each group). Results are expressed as mean +/- SEM. Each experiment was performed in duplicate. p values were determined by ANOVA. p < 0.05 was considered to be significant. FASN 5'/3', protocol A versus protocol B (p = 0.04), at any time of storage.

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