Table 1 Sequences in forward primers used for generation of the transcriptionally-regulatable PCR products
From: Cloning-free regulated monitoring of reporter and gene expression
SEQ | Forward Primer | PCR product will contain | I-TFS |
|---|---|---|---|
1 | 5' AGGGGACTTTCC TGAGTCAATAGGGACTTTCC AATGCCAAAATGTCGTAACAACTC3' | NF-κB responsive Minimal Promoter | NF-κB |
2 | 5' AGGCGACTCTCCTGAGTCAATACTCACTCT CCAATGCCAAAATGTCGTAACAACTC3' | Mutant NF-κB/Minimal Promoter | NF-κB mutant |
3 | 5'CAATAGGGACTTTCCCATGGGACTTTCCCAGGGGACTTTCCTGAGTCAATAGGGACTTTCCAATGGGTAGGCGTGTACGGTG3' | NF-κB (4×) responsive Minimal Promoter (-53) | NF-κB 4× |
4 | 5' CTGCGCTCAGGGGACCTTGCGCCCGGCCCTTCTGCTGCACACAGCCCACCCCAAAATGTCGTAACAACTC3' | Metal response Minimal Promoter | MT1F-MRE |
5 | 5'ACTCAGCGGGCTGGGTGCAAGGGCGGGGCGGGGCGTCTGCGCCCGGCCCCGCCAAAATGTCGTAACAACTC 3' | Metal response Minimal Promoter | MT1G-MRE |
6 | 5' AGCTTTAGTTTCAC TTTCCCCTTTCGGTTTC CCTAGGTTTCCAACCAATGCCAAAATGTCGTAACAACTC3' | IFN-responsive Promoter | ISRE |
7 | 5' CTTGGGGCGGGGCTGCGGAGATCTCGCGGCGCTTGGCGTGCTATAAAAGCAGAGCTCGTTTAGTGA 3' | RPS2 Minimal Promoter | - |
8 | 5' GAAGGCACTAGAAGAGGCGGTCGGTGCATAGCGTCACCTCCTAAACTCGCAGAGCTCGTTTAGTGA 3' | RPL39 Minimal Promoter | - |
9 | 5'GTAAATGACATAGGAAAACTGAAAGGGAGAAGTGAAAGTGGGAAATTCCTCTGAATAGAGAGAGGACCATCTCATATAAATAGCAGAGCTCGTTTAGTGA3' | Minimal virus-induced IFNB prom. | VRE |
10 | 5'ACCAGGTCCCTATCAGTGATAGAGATCCTCCCTTCAGTGATAGAGACCAAAATGTCGTAACAACTC3' | TetO2-Minimal Promoter (-95) | TetO |
11 | 5'ACCAGGTCCCTATCAGTGATAGAGATCCTCCCTATCAGTGATAGAGAGGTAGGCGTGTACGGTG3' | TetO2-Minimal Promoter (-53) | TetO |
12 | 5'CATCCCTATCAGTGATAGACCATCCCTATCAGTGATAGACCATCCCTATCAGTGATAGACGGTAGGC GTGTACGGTG3' | TetO3-Minimal Promoter (-53) | TetO |