TY - JOUR AU - al-Haj, Latifa AU - Al-Ahmadi, Wijdan AU - Al-Saif, Maher AU - Demirkaya, Omer AU - Khabar, Khalid SA PY - 2009 DA - 2009/03/08 TI - Cloning-free regulated monitoring of reporter and gene expression JO - BMC Molecular Biology SP - 20 VL - 10 IS - 1 AB - The majority of the promoters, their regulatory elements, and their variations in the human genome remain unknown. Reporter gene technology for transcriptional activity is a widely used tool for the study of promoter structure, gene regulation, and signaling pathways. Construction of transcriptional reporter vectors, including use of cis-acting sequences, requires cloning and time-demanding manipulations, particularly with introduced mutations. SN - 1471-2199 UR - https://doi.org/10.1186/1471-2199-10-20 DO - 10.1186/1471-2199-10-20 ID - al-Haj2009 ER -