Table 5 The primers used for the bisulfite sequencing of bPRP1.
Region | Strand | Sequence | Annealing |
|---|---|---|---|
Region 1 | Forward | 5'-GGGATTGTTGTTGTTGTTGTTAAGT-3' | 60 |
| Â | Reverse | 5'-AAAACTATCTCTTTCTCCATACTAATACCT-3' | Â |
Region 2 | Forward | 5'-TTGTGTATGAGATAGTAAAAGAGATATTGA-3' | 60 |
| Â | Reverse | 5'-CAATAAAAAACCAAAAAAACTATAATTACA-3' | Â |