Table 4 The primers used for the bisulfite sequencing of bCSH1.
Region | Strand | Sequence | Annealing |
|---|---|---|---|
Region 1 | Forward | 5'-AGGGAAGATTTTTTGGAGAAGG-3' | 60 |
| Â | Reverse | 5'-AATAATAACCTTCAAATAACCAATACAC-3' | Â |
Region 2 | Forward | 5'-AAGAGTTTGTATGGATTTTTTTAGA-3' | 60 |
| Â | Reverse | 5'-CCACACTCTTCCTCAATAATAATAA-3' | Â |
Region 3 | Forward | 5'-TTTGATAATTGTTTATTGAATGATTTATTA-3 | 60 |
| Â | Reverse | 5'-CAAATTCACTCCTAACCTATCTTTTCT-3' | Â |
Region 4 | Forward | 5'-AGTTTGTTAATAAATGAATTTTTTTT-3' | 56 |
| Â | Reverse | 5'-CTTACTTTTTCCTCTTTTCTACCCTAAA-3' | Â |
Region 5 | Forward | 5'-GTTTGGGGTTGAATATTTATTATT-3' | 60 |
| Â | Reverse | 5'-TACCTACTTCTATTTAATACCAATT-3' | Â |
Region 6 | Forward | 5'-ATGTTGTTTATTATAGGGTGTATA-3' | 56 |
| Â | Reverse | 5'-TTATATCTTTTACAATTTTAATACTAA-3' | Â |