Position | Strand | Sequence | Annealing |
---|---|---|---|
-860 to -845a | Forward | 5'-GGGAGCTCCCTGTAAAATATCATGTA-3' | 62 |
-510 to -495a | Forward | 5'-GGGAGCTCCCATTAATACCAACACAG-3' | 62 |
-277 to -262a | Forward | 5'-GGGAGCTCCCGACTCCTCCGCCCATG-3' | 62 |
-80 to -65b | Forward | 5'-GGGGTACCCCAGCTCTACTCCACAGG-3' | 65.5 |
-50 to -35b | Forward | 5'-GGGGTACCCCTTTTATGGCCTCATGG-3' | 65.5 |
+23 to +38c | Reverse | 5'-ATCTCGAGATGGGAATGCCTAAGGAG-3' | Â |