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Table 3 The primers used for the bPRP1 (AH001153) 5'-flanking region of the sequencing and reporter constructs.

From: The characterization of DNA methylation-mediated regulation of bovine placental lactogen and bovine prolactin-related protein-1 genes

Position

Strand

Sequence

Annealing

-860 to -845a

Forward

5'-GGGAGCTCCCTGTAAAATATCATGTA-3'

62

-510 to -495a

Forward

5'-GGGAGCTCCCATTAATACCAACACAG-3'

62

-277 to -262a

Forward

5'-GGGAGCTCCCGACTCCTCCGCCCATG-3'

62

-80 to -65b

Forward

5'-GGGGTACCCCAGCTCTACTCCACAGG-3'

65.5

-50 to -35b

Forward

5'-GGGGTACCCCTTTTATGGCCTCATGG-3'

65.5

+23 to +38c

Reverse

5'-ATCTCGAGATGGGAATGCCTAAGGAG-3'

 
  1. a The primers have a SacI recognition site (-GAGCTC-).b The primers have a KpnI recognition site (-GGTACC-). c The common reverse primers for all forward primers have an XhoI recognition site (-CTCGAG-) at their 5'-end.