Skip to main content

Table 1 The Oligonucleotide primers used for the RT-PCR and quantitative real-time RT-PCR.

From: The characterization of DNA methylation-mediated regulation of bovine placental lactogen and bovine prolactin-related protein-1 genes

Gene

Strand

Sequence

Position

RT-PCR

   

bCSH1

(NM_181007)

Forward

5'-CTGCTGGTGGTGTCAAATCTAC-3'

170–191

 

Reverse

5'-TGGTTGGGTTAATTGTGGGC-3'

828-812

bPRP1

(NM_174159)

Forward

5'-CACGGTCAACAGGAGTCCTCACC-3'

43–65

 

Reverse

5'-AATTTCAGGTAGCCCGCTGTGG-3'

873-852

GAPDH

(NM_001034034)

Forward

5'-CCTTCATTGACCTTCACTACATGGTCTA-3'

173–200

 

Reverse

5'-GCTGTAGCCAAATTCATTGTCGTACCA-3'

1029-1003

real-time RT-PCR

   

bCSH1

Forward

5'-GCAACATTGGTGGCTAGCAA-3'

281–300

 

Reverse

5'-GCCCTCGCCAAACTGTTTATTA-3'

358-337

bPRP1

Forward

5'-CACGGAGCTGCAGCATATGA-3'

501–520

 

Reverse

5'-CCTTGTGGCGCTTGATAGGA-3'

558-539

GAPDH

Forward

5'-AAGGCCATCACCATCTTCCA-3'

280–299

 

Reverse

5'-CCACCACATACTCAGCACCAGCAT-3'

355-332