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Table 5 Primer sequences and amplification summary

From: Selection of suitable housekeeping genes for expression analysis in glioblastoma using quantitative RT-PCR

Gene

Primer Sequence [5' → 3']

Amplicon size (bp)

Intervening sequence size (bp)

Amplification efficiency (%)

ACTB

F: GGCACCCAGCACAATGAAG

R: CCGATCCACACGGAGTACTTG

66

178

98

GAPDH

F: AGATCCCTCCAAAATCAAGTGG

R: GGCAGAGATGATGACCCTTTT

130

220

98

GUSB

F: GAAAATATGTGGTTGGAGAGCTCATT

R: CCGAGTGAAGATCCCCTTTTTA

101

3360

93

HMBS

F: CACGATCCCGAGACTCTGCT

R: TACTGGCACACTGCAGCCTC

81

315

104

HPRT1

F: TGAGGATTTGGAAAGGGTGT

R: GAGCACACAGAGGGCTACAA

118

1833

99

TBP

F: GAGCTGTGATGTGAAGTTTCC

R: TCTGGGTTTGATCATTCTGTAG

117

1747

110

18S rRNA

F: GGAGTATGGTTGCAAAGCTGA

R: ATCTGTCAATCCTGTCCGTGT

129

_

89

TG1

F: AAGGTCGGAAGAAGGTGACAG

R: GCTGCTGGAATTTGCTCACTG

120

4217

97

TG2

F: CTTTACCCAGCGACCGTTTCA

R: GGACTCAAGTAGGGCACAGAA

123

2206

96

TG3

F: TACTCTGATCGCTCCGTTAGG

R: CCTGCAAATGCCAGTGTCGTA

120

29982

92

TG4

F: AAAGATGCTGGGCAGTGCATC

R: CCACAGGAGTATCTTGCACAG

94

2754

90

TG5

F: GCCAACCCTTCGAAACATCTG

R: AGCTGCCAAAGTATTCACAGTC

130

242

110

TG6

F: AGCGCTGTGGTCGTTTGGAG

R: GACGAATAACCAGGTGGAGCT

132

231

101

TG7

F: TTGCCACAGGTGGGAAAGAGA

R: CAGTCATTCCGCACGTTCTTG

94

256

99

TG8

F: GAAGGGATGTACGTGTGACTC

R: CCATTCTCTGGGAGATGAAGC

131

2129

98

TG9

F: GCCGAAGCTTTGAACATGCAC

R: CACACTCAGCAACTGTCCTAG

93

4817

110

TG10

F: CTCTCCTGCTCTAGGATCAAG

R: ATTCCGCTGCTGACACCTAAC

124

3241

96

TG11

F: GAACAGTGGGCAGTGATTCAC

R: TTGGTGTCCTGTGGCTTAGTC

125

1351

87

TG12

F: CGGATTTCAGGAAGCCTTGTG

R: GCAGATGCTTGCACAGCATTC

131

4426

90

  1. F: forward primer, R: reverse primer