| Â | Â | Recovery Time |
|---|
mRNA | Transfected ODN | 48 hours | 72 hours |
|---|
| Â | Â | (Ct value) |
Dystrophin | mdx47NT | 34.1 ± 0.15 | 33.8 ± 0.22 |
|  | NS | 34.9 ± 0.08 | 37.1 ± 0.35 |
GAPDH | mdx47NT | 13.2 ± 0.08 | 13.6 ± 0.02 |
|  | NS | 13.3 ± 0.05 | 13.6 ± 0.32 |
CKM | mdx47NT | 22.1 ± 0.11 | 13.6 ± 0.11 |
|  | NS | 21.5 ± 0.08 | 13.6 ± 0.10 |
- A quantitative measurement of dystrophin transcripts containing the deleted portion of exon 10 was also performed. The cells were induced to differentiate with addition of media containing 2% horse serum at the indicated time points for 96 hours. Each sample was subjected to RT PCR using either dystrophin specific primers or oligo dT primers. QPCR was performed using a reverse primer specific for the deleted portion of exon 10. GAPDH was used as a loading control and CKM levels were measured to ensure similarities in the extent of differentiation between samples. Significance was measured by a Student's t-test and samples were considered significantly different with a p value of less than *0.05, **0.01, ***0.001. mdx47NT was used to correct the mutation while nonspecific ODN was used as a negative control. GAPDH and CKM were used as positive controls with the ODNs in parenthesis added to the mRNA mixtures.
