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Figure 1 | BMC Molecular Biology

Figure 1

From: Genetic correction of splice site mutation in purified and enriched myoblasts isolated from mdx5cv mice

Figure 1

Optimization of ODN uptake in primary myoblast cultures from mdx5cv mice. The percent of FAM labeled ODN uptake complexed with the transfection reagent jetPEI (blue bars) as a function of the seeding density of the myoblasts is shown as well as the amount of background fluorescence when myoblasts are incubated with either the FAM oligo or jetPEI alone. The cell density is the 2.5 × 106 when the cells are treated with FAM or jetPEI alone. The amount of cell death at each cell density and control samples is also shown (red bars).

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