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Table 2 Strains

From: Expansion of a chromosomal repeat in Escherichia coli: roles of replication, repair, and recombination functions

Strain

Relevant Genotype

Reference, source, or construction

FC691

lacIZ33a

[38]

GM3819

damΔkan

M. Marinus

GM8291

polAΔfrt-kan/F' polA+ camR

M. Marinus; polA allele [39]

MG1655

wild type

 

MV1132

srl300::Tn10 recAo281

M. Volkert

MV1154

lexA3

M. Volkert

MV2104

lexA71::Tn5

M. Volkert

TP538

recGΔtet

[40]

TP539

recGΔkan

[40]

TP540

ruvABΔtet

[40]

TP547

red-catb

derivative of KM32 [41]

TP577

recFΔtet

[40]

TP605

sulAΔtet

[40]

TP643

recQΔtet

[24]

TP645

recRΔtet

[24]

TP662

sulAΔkan

substitution of Tn903 aph for Tn10 tetRA in sulAΔtet [40]

TP664

recNΔtet

[42]

TP730

red-cat lacIZ33

FC691 × P1•TP547

TP732

red-pae-cIb lacIZ33

TP730 × pTP822 linear [41]

TP796

recAΔtet

[43]

TP797

ruvCΔtet

[43]

TP798

red-cat

[36]

TP832

red-ampb

[36]

TP838

recBCDΔtet

[43]

TP872

red-amp lacΔcat

TP832 × cat15,16 pcr of Tn9c

TP889

lacΔcat

MG1655 × P1•TP872

TP922

red-pae-cIb (lacIZ33Y)2-tet-oriR6Kãd

TP732 × pTP1061 lineare

TP929

(lacIZ33Y)2-tet-oriR6Kgamma

TP889 × P1•TP922

TP942

red-pae-cI (lacIZ33Y)2-cat

TP922 × Toc1,2 pcr of Tn9

TP997

galK::aacC1067

[44]

TP1000

dinBΔtet

TP798 × din7,8 pcr of Tn10

TP1003

lacΔspc

TP889 was transduced with a P1 lysate of an unnamed intermediate strain, TP798 × LAT2,3 pcr of Tn21 aadA

TP1004

(lacIZ33Y)2-cat

TP929 × P1•TP942

TP1005

(lacIZ33Y)2-cat dinBΔtet

TP1004 × P1•TP1000

TP1006

(lacIZ33Y)2-cat recAΔtet

TP1004 × P1•TP796

TP1007

(lacIZ33Y)2-cat recFΔtet

TP1004 × P1•TP577

TP1008

(lacIZ33Y)2-cat recGΔtet

TP1004 × P1•TP538

TP1009

(lacIZ33Y)2-cat recNΔtet

TP1004 × P1•TP664

TP1011

(lacIZ33Y)2-cat recQΔtet

TP1004 × P1•TP643

TP1012

(lacIZ33Y)2-cat recBCDΔtet

TP1004 × P1•TP838

TP1014

(lacIZ33Y)2-cat ruvABΔtet

TP1004 × P1•TP540

TP1015

(lacIZ33Y)2-cat ruvCΔtet

TP1004 × P1•TP797

TP1020

(lacIZ33Y)2-cat recRΔtet

TP1004 × P1•TP645

TP1022

lacIZ33

Spontaneous chloramphenicol-sensitive TP1004 derivative

TP1031

red-cat recJΔtet

TP798 × recJ1,2 pcr of Tn10

TP1032

red-cat rhnAΔtet

TP798 × rnhA1,2 pcr of Tn10

TP1033

red-cat polBΔtet

TP798 × polB1,2 pcr of Tn10

TP1034

(lacIZ33Y)2-cat recJΔtet

TP1004 × P1•TP1031

TP1035

(lacIZ33Y)2-cat rhnAΔtet

TP1004 × P1•TP1032

TP1036

(lacIZ33Y)2-cat polBΔtet

TP1004 × P1•TP1033

TP1038

red-amp (lacIZ33Y)2-cat

TP832 × P1•TP942

TP1042

(lacIZ33Y)2-cat damΔkan

TP1004 × P1•GM3819

TP1043

(lacIZ33Y)2-cat sulAΔtet

TP1004 × P1•TP605

TP1047

(lacIZ33Y)2-cat uvrDΔtet

TP1004/pKM208 × uvrD1,2 pcr of Tn10

TP1048

(lacIZ33Y)2-cat sulAΔtet lexA71::Tn5

TP1043 × P1•MV2104

TP1049

(lacIZ33Y)2-cat umuDCΔtet

TP1004/pKM208 [45] × umDC1,2 pcr of Tn10

TP1050

(lacIZ33Y)2-cat malFΔspc

TP1004/pKM208 × malF1,2 pcr of TP997

TP1051

(lacIZ33Y)2-cat rpoSΔtet

TP1004/pKM208 × rpoS1,2 pcr of Tn10

TP1053

(lacIZ33Y)2-cat adaΔkan

TP1004/pKM208 × ADA1,2 pcr of Tn903 aph

TP1054

(lacIZ33Y)2-cat priAΔkan

TP1004/pKM208 × priA1,2 pcr of Tn903 aph

TP1055

(lacIZ33Y)2-cat relAΔkan

TP1004/pKM208 × relA1,2 pcr of Tn903 aph

TP1056

(lacIZ33Y)2-cat sulAΔtet priAΔkan

TP1043 × P1•1054

TP1057

red-amp (lacIZ33Y)2-cat recAΔtet

TP1038 × P1•796

TP1058

red-amp (lacIZ33Y)2-cat recFΔtet

TP1038 × P1•577

TP1059

red-amp (lacIZ33Y)2-cat recGΔtet

TP1038 × P1•538

TP1061

red-amp (lacIZ33Y)2-cat rnhAΔtet

TP1038 × P1•1035

TP1062

lacIZ33 recAΔtet

TP1022 × P1•796

TP1063

(lacIZ33Y)2-cat recAΔkan

TP1004/pKM208 × recA3,4 pcr of Tn903 aph

TP1064

(lacIZ33Y)2-cat mutHΔtet

TP1004/pKM208 × mutH1,2 pcr of 1016

TP1065

(lacIZ33Y)2-cat mutMΔtet

TP1004/pKM208 × mutM1,2 pcr of Tn10

TP1066

(lacIZ33Y)2-cat mutYΔkan

TP1004/pKM208 × mutY1,2 pcr of Tn903 aph

TP1069

(lacIZ33Y)2-cat mutMΔtet mutYΔkan

TP1065 × P1TP1066

TP1080

lacΔcat recAΔtet

TP889 × P1•TP796

TP1081

lacΔcat recBCDΔtet

TP889 × P1•TP838

TP1082

lacΔcat ruvCΔtet

TP889 × P1•TP797

TP1086

(lacIZ33Y)2-cat polAΔfrt-kan

TP1004 × P1•JW3835

TP1089

(lacIZ33Y)2-cat yfgLΔkan

TP1004/pKM208 × yfgL1,2 pcr of Tn903 aph

TP1090

(lacIZ33Y)2-cat lexA3

TP1050 × P1•MV1154, selection for Mal+, screen for UV-sensitivity

TP1091

(lacIZ33Y)2-cat damΔkan mutHΔtet

TP1064 × P1•GM3819

TP1095

(lacIZ33Y)2-cat srl300::Tn10 recAo281

TP1063 × P1•MV1132

TP1098

(lacIZ33Y)2-cat ruvCΔtet recGΔkan

TP1015 × P1•TP539

TP1099

(lacIZ33Y)2-cat recAo281

TP1095 × P1•TP1063, selection for Srl+, screen for kanamycin sensitivity and the Sph site created by the recAo281 mutation [46]

  1. Notes
  2. a. The abbreviation lacIZ33 is used to denote the triple mutant Ï•(lacI33-lacZ) bearing the lacIQpromoter-up mutation in the lacI promoter, a 212-bp deletion fusing lacI to lacZ, and a +1 frameshift mutation in the lacI sequences near the fusion junction [13].
  3. b. Strains designated "red" bear the phage λ red recombination genes, which replace the recC-ptr-recB-recD gene cluster in the E. coli chromosome.
  4. c. Primers used in polymerase chain reactions to generate antibiotic cassettes for gene replacements are described in Table 3.
  5. d. Strains designated "(lacIZ33Y)2-" bear a duplication of lacIZ33 and lacY, with the genetic element listed after the hyphen (tet-oriR6Kgamma or cat) inserted between the two copies.
  6. e. Details of this construction are given in the text.
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BMC Molecular Biology

ISSN: 1471-2199

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