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Table 4 Schedule of tick feeding employed for the synchronous feeding of all life stages from both R. microplus and R. appendiculatus on calf 1471.

From: Selection of reference genes for quantitative RT-PCR studies in Rhipicephalus (Boophilus) microplus and Rhipicephalus appendiculatus ticks and determination of the expression profile of Bm86

Action

Species

Time point (days)

Collection of eggs

R. microplus

4, 10, 15, 20, 22 and 24 days post-oviposition

 

R. appendiculatus

20 days post-oviposition

Collection of unfed larvae

R. microplus &

R. appendiculatus

21 days post-hatching (45 days post-oviposition)

Placement of unfed larvae

R. microplus &

R. appendiculatus

0

Collection of partially fed larvae

R. microplus &

R. appendiculatus

4

Collection of pharate nymphs

R. microplus

R. appendiculatus

6

7 days post-engorgement

Collection of unfed nymphs

R. microplus

R. appendiculatus

7

21 days post-molting

Placement of unfed nymphs

R. appendiculatus

7

Collection of partially fed nymphs

R. microplus &

R. appendiculatus

11

Collection of pharate adults

R. microplus

R. appendiculatus

13

7 days post engorgement

Collection of unfed males

R. microplus

R. appendiculatus

14

21 days post-molting

Collection of unfed females

R. microplus

R. appendiculatus

15

21 days post-molting

Placement of unfed adults

R. appendiculatus

15

Collection of partially fed adults

R. microplus &

R. appendiculatus

22