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Table 4 Schedule of tick feeding employed for the synchronous feeding of all life stages from both R. microplus and R. appendiculatus on calf 1471.

From: Selection of reference genes for quantitative RT-PCR studies in Rhipicephalus (Boophilus) microplus and Rhipicephalus appendiculatus ticks and determination of the expression profile of Bm86

Action Species Time point (days)
Collection of eggs R. microplus 4, 10, 15, 20, 22 and 24 days post-oviposition
  R. appendiculatus 20 days post-oviposition
Collection of unfed larvae R. microplus &
R. appendiculatus
21 days post-hatching (45 days post-oviposition)
Placement of unfed larvae R. microplus &
R. appendiculatus
0
Collection of partially fed larvae R. microplus &
R. appendiculatus
4
Collection of pharate nymphs R. microplus
R. appendiculatus
6
7 days post-engorgement
Collection of unfed nymphs R. microplus
R. appendiculatus
7
21 days post-molting
Placement of unfed nymphs R. appendiculatus 7
Collection of partially fed nymphs R. microplus &
R. appendiculatus
11
Collection of pharate adults R. microplus
R. appendiculatus
13
7 days post engorgement
Collection of unfed males R. microplus
R. appendiculatus
14
21 days post-molting
Collection of unfed females R. microplus
R. appendiculatus
15
21 days post-molting
Placement of unfed adults R. appendiculatus 15
Collection of partially fed adults R. microplus &
R. appendiculatus
22