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Table 3 Candidate reference genes ranked according to their expression stability as calculated by the Normfinder and geNorm programs.

From: Selection of reference genes for quantitative RT-PCR studies in Rhipicephalus (Boophilus) microplus and Rhipicephalus appendiculatus ticks and determination of the expression profile of Bm86

R. microplus R. appendiculatus R. microplus and R. appendiculatus
geNorm Normfinder geNorm Normfinder geNorm Normfinder
ELF1A and RPL4 (0.301) TBP (0.400) ELF1A and RPL4 (0.371) GAPDH (0.359) ELF1A and RPL4 (0.376) ELF1A (0.477)
  ELF1A (0.459)   ELF1A (0.424)   GAPDH (0.521)
H3F3A (0.559) RPL4 (0.463) TBP (0.614) RPL4 (0.481) TBP (0.619) TBP (0.549)
TBP (0.656) BTUB (0.485) GAPDH (0.764) PPIA (0.580) H3F3A (0.778) PPIA (0.555)
BTUB (0.771) PPIA (0.512) H3F3A (0.856) TBP (0.583) PPIA (0.926) RPL4 (0.563)
PPIA (0.824) H3F3A (0.577) PPIA (0.959) H3F3A (0.709) GAPDH (0.995) H3F3A (0.623)
GAPDH (0.940) GAPDH (0.649) ACTB (1.097) ACTB (0.720) BTUB (1.074) BTUB (0.680)
ACTB (1.159) ACTB (0.952) BTUB (1.203) BTUB (0.852) ACTB (1.259) ACTB (0.962)
GST (1.512) GST (1.450) GST (1.818) GST (2.128) GST (1.795) GST (1.899)
  1. The candidates are listed with decreasing expression stability from top to bottom. Average expression stability values are shown between parentheses.