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Table 2 Cycle threshold (Ct) values of candidate reference genes and Bm86

From: Selection of reference genes for quantitative RT-PCR studies in Rhipicephalus (Boophilus) microplus and Rhipicephalus appendiculatus ticks and determination of the expression profile of Bm86

Gene Ct Range Ct Min. Ct Max mean Ct ± s.e.m.
  Rm a Ra a Rm a Ra a Rm a Ra a Rm a Ra a
ACTB 5.62 5.92 14.85 11.80 20.47 17.72 16.48 ± 0.25 15.59 ± 0.24
BTUB 4.01 4.74 19.57 19.22 23.58 23.96 21.00 ± 0.16 21.76 ± 0.24
ELF1A 2.36 3.90 16.36 15.34 18.72 19.24 17.29 ± 0.12 17.01 ± 0.17
GAPDH 4.39 3.47 21.67 20.56 26.06 24.04 23.02 ± 0.20 22.29 ± 0.17
GST 10.77 13.41 20.28 20.90 31.05 34.31 24.27 ± 0.51 27.22 ± 0.67
H3F3A 4.03 4.69 16.35 17.68 20.39 22.36 18.74 ± 0.15 19.90 ± 0.20
PPIA 4.38 3.90 17.92 17.98 22.30 21.88 19.31 ± 0.20 19.91 ± 0.20
RPL4 3.09 3.83 17.68 17.39 20.77 21.22 18.82 ± 0.12 18.87 ± 0.15
TBP 2.27 4.28 25.74 25.70 28.01 29.98 27.01 ± 0.09 26.99 ± 0.20
Bm86 10.38 7.56 21.28 19.86 31.66 27.42 24.05 ± 0.40 22.85 ± 0.44
  1. aRm, R. microplus; Ra, R. appendiculatus