Selection of reference genes for quantitative RT-PCR studies in Rhipicephalus (Boophilus) microplus and Rhipicephalus appendiculatus ticks and determination of the expression profile of Bm86

Table 2 Cycle threshold (C_t) values of candidate reference genes and Bm86

Gene	C_t Range		C_t Min.		C_t Max		mean C_t ± s.e.m.
	Rm ^a	Ra ^a	Rm ^a	Ra ^a	Rm ^a	Ra ^a	Rm ^a	Ra ^a
ACTB	5.62	5.92	14.85	11.80	20.47	17.72	16.48 ± 0.25	15.59 ± 0.24
BTUB	4.01	4.74	19.57	19.22	23.58	23.96	21.00 ± 0.16	21.76 ± 0.24
ELF1A	2.36	3.90	16.36	15.34	18.72	19.24	17.29 ± 0.12	17.01 ± 0.17
GAPDH	4.39	3.47	21.67	20.56	26.06	24.04	23.02 ± 0.20	22.29 ± 0.17
GST	10.77	13.41	20.28	20.90	31.05	34.31	24.27 ± 0.51	27.22 ± 0.67
H3F3A	4.03	4.69	16.35	17.68	20.39	22.36	18.74 ± 0.15	19.90 ± 0.20
PPIA	4.38	3.90	17.92	17.98	22.30	21.88	19.31 ± 0.20	19.91 ± 0.20
RPL4	3.09	3.83	17.68	17.39	20.77	21.22	18.82 ± 0.12	18.87 ± 0.15
TBP	2.27	4.28	25.74	25.70	28.01	29.98	27.01 ± 0.09	26.99 ± 0.20
Bm86	10.38	7.56	21.28	19.86	31.66	27.42	24.05 ± 0.40	22.85 ± 0.44

ISSN: 1471-2199