Selection of reference genes for quantitative RT-PCR studies in Rhipicephalus (Boophilus) microplus and Rhipicephalus appendiculatus ticks and determination of the expression profile of Bm86

Table 1 Details of the quantitative RT-PCRs of Bm86 and the candidate reference genes evaluated in this study.

Symbol	Gene name	Function	GenBank accession number		Forward primer	Reverse primer	Amplicon length	Efficiency
			Rm ^a	Ra ^a				Rm ^a	Ra ^a
ACTB	Beta actin	Cytoskeletal structural protein	AY255624	AY254899	CCCATCTACGAAGGTTACGCC	CGCACGATTTCACGCTCAG	139 bp	102	98
Bm86	Bm86	Unknown	FJ809946	FJ809944	CGTCCCGACTTGACCTGC	AGGAGCGGCTGAACAGTTTG	101 bp	103	103
BTUB	Beta tubulin	Component of microtubules	CK179480	CD781348	AACATGGTGCCCTTCCCACG	GCAGCCATCATGTTCTTTGC	140 bp	92	97
ELF1A	Elongation factor 1-alpha	Component of the eukaryotic translational apparatus	EW679365	CD797149	CGTCTACAAGATTGGTGGCATT	CTCAGTGGTCAGGTTGGCAG	108 bp	100	92
GAPDH	Glyceraldehyde-3-phosphate dehydrogenase	Oxireductase in glycolysis and gluconeogenesis	CK180824	CD791831	AGTCCACCGGCGTCTTCCTCA	GTGTGGTTCACACCCATCACAA	123 bp	97	91
GST	Glutathione S-transferase	Detoxification of endobiotic and xenobiotic substrates	CV456312	CD789942	TACCTGGGCAAGAAGCACGG	AGAGCCCAGAGCAGGTCGTTG	98 bp	93	100
H3F3A	H3 Histone family 3A	Involved in structure of chromatin	CV442167	CD795637	AAGCAGACCGCCCGTAAGT	GTAACGACGGATCTCCCTGAG	152 bp	92	95
PPIA	Cyclophilin	Facilitate protein folding	CV445080	CD793819	CTGGGACGGATAGTAATTGAGC	ATGAAGTTGGGGATGACGC	133 bp	95	98
RPL4	Ribosomal protein L4	Structural component of the large 60S ribosomal subunit	CV447629	CD794864	AGGTTCCCCTGGTGGTGAG	GTTCCTCATCTTTCCCTTGCC	152 bp	93	98
TBP	TATA box binding protein	Transcription factor	CV453818	CD780134	CTTGTCCTCACACACAGCCAGTT	GTGAGCACGACTTTTCCAGATAC	122 bp	94	100

ISSN: 1471-2199