BMC Molecular Biology

Table 2 Detection of ER specific genes by RT-PCR on total cDNA before and after SCOTS

From: Innovative approach for transcriptomic analysis of obligate intracellular pathogen: selective capture of transcribed sequences of Ehrlichia ruminantium

Gene	24 hpi				72 hpi				96 hpi				120 hpi
	0×	1×	2×	3×	0×	1×	2×	3×	0×	1×	2×	3×	0×	1×	2×	3×
map 1	-	+	++	+++	-	+	++	+++	-	+	++	+++	-	+	++	+++
recA 1	-	-	-	+	-	+	++	+++	-	-	++	+++	-	-	-	+++
rpo D	-	-	+	++	-	+	++	+++	-	-	++	+++	-	-	-	+++
ffh	-	+	++	+++	-	+	++	+++	-	-	++	+++	-	-	++	+++
pCS20	-	+	+	+	-	+	+	+	-	+	++	+++	-	+	++	+++

-: absence of amplicon after RT-PCR, +: Presence of amplicon after RT-PCR.
The number of (+) corresponded to the intensity of amplification signal. 0×, 1×, 2× and 3× corresponded to results obtained on cDNA before and after one, two and three rounds of capture respectively. hpi: hours post infection

Back to article page

ISSN: 1471-2199

Contact us

General enquiries: ORSupport@springernature.com