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Table 2 Detection of ER specific genes by RT-PCR on total cDNA before and after SCOTS

From: Innovative approach for transcriptomic analysis of obligate intracellular pathogen: selective capture of transcribed sequences of Ehrlichia ruminantium

Gene 24 hpi 72 hpi 96 hpi 120 hpi
 
map 1 - + ++ +++ - + ++ +++ - + ++ +++ - + ++ +++
recA 1 - - - + - + ++ +++ - - ++ +++ - - - +++
rpo D - - + ++ - + ++ +++ - - ++ +++ - - - +++
ffh - + ++ +++ - + ++ +++ - - ++ +++ - - ++ +++
pCS20 - + + + - + + + - + ++ +++ - + ++ +++
  1. -: absence of amplicon after RT-PCR, +: Presence of amplicon after RT-PCR.
  2. The number of (+) corresponded to the intensity of amplification signal. 0×, 1×, 2× and 3× corresponded to results obtained on cDNA before and after one, two and three rounds of capture respectively. hpi: hours post infection