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Table 3 Coefficient of Variationa (CV) and Ct difference (ΔCt = Ctmax-Ctmin) determined on raw Ct values obtained by 18 primer pairs which amplified specific or multiple (m) gene transcripts of four gene families (Actin, α-tubulin, Translation elongation factor and ADP-ribosylation factor) in five data-sets.

From: Identification and validation of reference genes for quantitative RT-PCR normalization in wheat

Unigene cluster Gene annotation I Data setb II Data setc III Data setd IV Data sete V Data setf
   CV ΔCt CV ΔCt CV ΔCt CV ΔCt CV ΔCt
Ta54225 Actin 3.87 3.01 4.14 2.90 1.83 1.21 1.99 1.14 2.95 1.57
Actin (m) Actin 4.41 3.78 4.46 3.78 3.87 1.90 1.27 0.65 3.12 1.76
Ta1868 Actin 5.37 4.81 5.87 4.81 2.10 1.26 3.28 2.36 2.67 1.96
Ta4344 Actin 6.09 6.15 6.90 6.15 1.73 1.12 4.35 2.58 6.71 4.97
Ta20863 Actin 6.65 6.50 7.26 6.65 3.03 2.40 3.81 2.43 7.56 6.42
Ta53908 Actin 7.20 6.90 7.92 6.90 2.67 1.94 3.70 2.23 8.20 6.21
Ta25534 α-tubulin 5 4.65 4.14 4.79 4.14 3.16 2.25 2.75 1.90 2.98 2.31
Ta54519 α-tubulin 4 6.94 7.33 7.83 7.33 4.03 2.82 5.65 3.70 7.04 5.38
α-tubulin (m) α-tubulin 7.01 6.42 7.82 6.42 3.31 1.63 3.84 1.61 8.25 5.41
Ta53981 α-tubulin 2 7.54 6.39 8.31 6.39 4.00 2.04 3.52 1.93 9.21 6.25
Ta33558 α-tubulin 3 7.77 7.02 8.59 7.02 4.09 2.56 5.21 3.52 9.37 7.02
Ta5566 α-tubulin 1 8.05 8.49 8.82 8.49 4.84 2.73 5.42 3.58 8.04 6.60
Ta53964 Translation EF 1α-subunit 3.55 3.46 3.74 3.46 1.04 0.66 1.74 0.91 2.71 2.00
TEF-1α (m) Translation EF 1α-subunit 4.53 4.09 5.08 4.09 2.20 1.17 2.77 1.49 4.01 2.60
Ta659 Translation EF 1α-subunit 4.82 4.35 5.27 4.35 2.12 1.16 2.50 1.22 3.82 2.48
Ta2291 ADP-ribosylation factor 4.54 3.46 4.85 3.46 2.02 1.27 1.38 0.70 3.86 2.55
Ta45379 ADP-ribosylation factor 4.59 4.03 4.85 4.03 2.21 1.35 2.15 1.18 5.16 3.77
ADP-RF (m) ADP-ribosylation factor 6.70 4.68 6.24 4.68 3.73 2.23 2.22 1.19 6.26 4.34
  1. aCV = Standard deviation divided by mean and multiplied by 100 of MST (Mean Square Total) among tissues, biological experiments and technical replicates within each of the two experiments.
  2. bAll the 24 samples including tissues, developmental stages and temperature treatments.
  3. c18 tissues and developmental stages.
  4. dSix samples consisting of two temperature treatments (4°C and 33°C) for 24 and 48 h and their controls.
  5. eSix floral organs from fully emerged spikes.
  6. fSix vegetative tissues and developmental stages (shoots, stems and leaves).