Relative quantification of TaPDIL1-1 transcripts in three data sets using selected reference genes as internal controls. A) Six vegetative tissues and shoot developmental stages (shoots, stems and leaves); B) six floral organs from fully emerged spikes; C) six samples consisting of two temperature treatments (4°C and 33°C) for 24 and 48 h and their controls (LT = low temperature; HT = high temperature). The TaPDIL1-1 expression levels were normalized using the five methods described in Fig. 5. Normalized values of TaPDIL1-1 relative expression are given as average ± SD.