Figure 1
From: Identification and validation of reference genes for quantitative RT-PCR normalization in wheat
Specificity of qRT-PCR amplification. (A) Dissociation curves of six representative reference genes showing single peaks (each including three technical replicates for each of 18 cDNA pools from different tissues and developmental stages of wheat). (B) Agarose gel (2%) showing amplification of a specific PCR product of the expected size for the same genes in (A). Dissociation curves and agarose gels for all the 42 candidate reference genes selected are shown in Additional file 8.