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Figure 2 | BMC Molecular Biology

Figure 2

From: Nuclear factor I-A represses expression of the cell adhesion molecule L1

Figure 2

Expression of HA-tagged NFI-A isoforms in CHO cells. A, Schematic drawing of the brain-specific isoform ("NFI-A bs") and the ubiquitous "standard" isoform ("NFI-A st") of mouse NFI-A. The isoform-specific N-termini (open rectangles) are encoded by exons 1a and 1b, respectively, and result from alternative splicing. Amino acid sequences encoded by these exons are given above and below. The major part of the protein (filled rectangles; not drawn to scale) is identical in both isoforms. Total length: 532 amino acids (NFI-A bs); 509 amino acids (NFI-A st). B, CHO cells were transfected with 2 μg of NFI-A cDNA expression vectors. Lane 1, pCHNFI-A ("standard" isoform); lane 2, pCHBNFI-A (brain-specific isoform); lane 3, mock transfected. Whole cell extracts were analyzed on an 8% SDS-PAGE gel, transferred to nitrocellulose membrane, and probed with anti-HA antibody. Filled arrows indicate bands which correspond to the respective HA-tagged NFI-A polypeptides, the open arrow shows a nonspecific band.

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