Figure 3

Splicing profile of endogenous VEGF gene andtransfected with pGFP-E6A plasmids in HEK293 and BT474 cells. A. mRNA for endogenous human VEGF isoforms were amplified using specific primers on mRNA extracted from of HEK293 and BT474 cells. The expected sizes of the products are indicated on the right. B. HEK293 and BT474 cells in triplicate were transfected with pGFP-E6A. After 48 hr, total RNA were assessed using RT-PCR. The anticipated positions of the products from the splicing events with inclusion or exclusion of exon 6A are indicated by arrows. C. Comparison of Exon 6A inclusion rate for endogenous and transfected reporter minigene between HEK293 cells and BT474 cells. The average ± standard error for three experiments is shown.