Effect of Sl SCP-x and Sl SCPx-2 protein expression on the absorption of cholesterol in the Spli-221 cells transfected with pE Sl SCPx/GFP and pE Sl SCPx-2/GFP transfection vectors, respectively. (A) Western blotting analysis of the protein extracts from the transfected Spli-221 cells. Proteins were analyzed on a 12% SDS-PAGE gel and each lane contained 20 μg protein. (B) Cholesterol concentrations in the transfected cells were analyzed using HPLC at 36 h post transfection. The control was the cells transfected with a pEGFP vector expressing only GFP. Three independent replicates were performed for each data point. The concentration of cholesterol in the cells or hemolymph was calculated as μg/ml of medium or hemolymph.