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Figure 7 | BMC Molecular Biology

Figure 7

From: The sterol carrier protein 2/3-oxoacyl-CoA thiolase (SCPx) is involved in cholesterol uptake in the midgut of Spodoptera litura: gene cloning, expression, localization and functional analyses

Figure 7

Effect of Sl SCP-x and Sl SCPx-2 protein expression on the absorption of cholesterol in the Spli-221 cells transfected with pE Sl SCPx/GFP and pE Sl SCPx-2/GFP transfection vectors, respectively. (A) Western blotting analysis of the protein extracts from the transfected Spli-221 cells. Proteins were analyzed on a 12% SDS-PAGE gel and each lane contained 20 μg protein. (B) Cholesterol concentrations in the transfected cells were analyzed using HPLC at 36 h post transfection. The control was the cells transfected with a pEGFP vector expressing only GFP. Three independent replicates were performed for each data point. The concentration of cholesterol in the cells or hemolymph was calculated as μg/ml of medium or hemolymph.

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