RT-PCR amplification strategy of S. litura Sl SCPx cDNA from mRNA. (A) RT-PCR amplification strategy, PCR-amplified products (B) and diagram of alternative splicing of Sl SCPx transcripts (C). The positions of the forward and reverse primers for RT-PCR are indicated in arrows below the sequence diagram. The arrows above the sequence diagram show the position of primers for the Sl SCPx dsRNA preparation (A). The two PCR products were amplified and their sizes were 1.6 kb for the full-length Sl SCPx and 0.6 kb for the Sl SCPx-C region, respectively (B). The dotted line shows the deleted Sl SCPx-t fragmentfrom the N-terminal (43 to 1,168 nucleotides) of Sl SCPx sequence, as compared to Sl SCPx-2.