Figure 6

Transactivation of HRE-luciferase activity by gcHIF-1 is inhibited by gcCITED3a and gcCITED3b. CHO cells were co-transfected with a pSV40-EpoHRE-luciferase reporter and pSV-β-gal vector (control for transfection efficiency) along with pBK-CMV-gcHIF-1α and pBK-CMV-gcARNT2b or empty pCMV-TNT vector. Wild-type pCMV-gcCITED3a or deletion mutant plasmid pCMV-gcCITED3aΔCR2 (panel A); or pCMV-gcCITED3b or deletion mutant plasmid pCMV-gcCITED3bΔCR2 (panel B) was also co-transfected with pBK-CMV-gcHIF-1α. Relative luciferase activity is the ratio of luciferase over β-galactosidase activity. Transfections were performed in triplicates and data are expressed as means ± S.D. of three independent experiments. Single asterisks (*) indicate significant differences between CHO cells co-transfected with (filled bars) or without (open bars) gcHIF-1 in the absence of gcCITED, p < 0.05; double asterisks (**) indicate significant differences in luciferase activities of CHO cells co-transfected with pCMV-gcCITED or pCMV-gcCITEDΔCR2 in the presence of gcHIF-1, p < 0.05.