Figure 5

ChIP-PCR analysis of gcHIF-1 binding to the gcCited3a and gcCited3b gene promoters. A. The primer-sets (indicated by opposing arrows of the same grey scale) for ChIP-PCR amplification of different regions of the 5'-flanking sequence of gcCITED3a are shown, and the regions analyzed are demarcated on the right panel. B. The primer-sets (indicated by opposing arrows of the same grey scale) for ChIP-PCR amplification of different regions of the 5'-flanking sequence of gcCITED3b are shown, and the regions analyzed are demarcated on the right panel. Representative ChIP results from kidney (upper panel) and liver (lower panel) of normoxic (left panel) and hypoxic (right panel) fish. The PCR products of samples without immunoprecipitation (Input), immunoprecipitated with anti-gcHIF-1α anti-serum (IP) and water (-ve) were resolved by gel electrophoresis. A PCR band appears if a DNA region is bound by gcHIF-1 in the IP (immunoprecipitated) samples.