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Figure 4 | BMC Molecular Biology

Figure 4

From: Functional characterization of two CITED3 homologs (gcCITED3a and gcCITED3b) in the hypoxia-tolerant grass carp, Ctenopharyngodon idellus

Figure 4

Transcriptional activation of gcCited3a and gcCited3b gene promoters and identification of gcHIF-1 binding sites by ChIP-PCR. CHO cells were transiently transfected with the pCITED3a(-1817/+30), pCITED3b(-1713/+76) or pGL3-Basic luciferase vectors together with pCMV-gcHIF1α, pCMV-gcARNT2b (HIF-1 complex) and pSVβ-gal plasmid or the empty pCMV vector. Luciferase was normalized against β-gal activity and data represent the means ± S.D. of three independent experiments. Asterisks indicate significant differences, P ≤ 0.05, between pCITED3a and pCITED3b promoter constructs and the pGL3-Basic vector.

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