Figure 3From: Functional characterization of two CITED3 homologs (gcCITED3a and gcCITED3b) in the hypoxia-tolerant grass carp, Ctenopharyngodon idellusNorthern blot analysis of gcCited3a and gcCited3b. Fish tissues (brain, gill, heart, kidney, liver, and muscle) were isolated from normoxic (N) and hypoxic (H) grass carp following 4 h and 96 h of exposure as indicated. Total RNA from each tissue was isolated and analyzed by Northern hybridization using gene-specific probes for gcCITED3a and gcCITED3b. Ethidium bromide-stained 28S rRNA is shown as the loading control. A representative Northern blot derived from the tissues of one normoxic and one hypoxic fish (from a total of three in each group) is shown here.Back to article page