Evaluation of real-time PCR endogenous control genes for analysis of gene expression in bovine endometrium

BMC Molecular Biology

Table 3 GeNorm output used to determine the optimal number of endogenous control genes for normalisation in bovine endometrial tissue samples.

V2/3	V3/4	V4/5	V5/6	V6/7	V7/8	V8/9	V9/10	V10/11	V11/12	V12/13	V13/14	V14/15
0.04	0.03	0.03	0.02	0.02	0.02	0.02	0.02	0.02	0.02AAY	0.02	0.02	0.01

GeNorm calculates the minimum number of genes required for accurate normalisation. In this case, two genes were sufficient (V2/3 = 0.04). The number of genes necessary for accurate normalisation is defined as the point at which the addition of a gene has a non-significant effect on the calculation of the normalisation factor, the threshold for a non-significant difference being 0.15.

ISSN: 1471-2199