Characterization of a novel bifunctional mutant of staphylokinase with platelet-targeted thrombolysis and antiplatelet aggregation activities
© Chen et al.; licensee BioMed Central Ltd. 2007
Received: 13 January 2007
Accepted: 07 October 2007
Published: 07 October 2007
Although staphylokianse (SAK) is among the most promising blood dissolving agents, it is far from ideal. It is interesting to hypothesize that the clot lysis efficacy of SAK can be enhanced with direct active platelet binding ability, and at the same time the rethrombosis complication after successful recanalization can be minimized with an antiplatelet aggregation activity. The present study was performed to characterize the functional properties of RGD-SAK, a novel mutant of staphylokinase (SAK).
By using site-directed mutagenesis, an Arg-Gly-Asp (RGD) motif was engineered in the staphylokinase (SAK). This mutant of SAK designated RGD-SAK was expressed, purified and characterized. Biochemical analysis indicated that RGD-SAK maintained the similar structure and the fibrinolytic function of SAK. Measurement of platelet binding activity in vitro demonstrated that RGD-SAK had a much higher affinity with platelets than SAK. In vitro platelet-rich clot lysis assay demonstrated that the engineered mutant outperformed the non-manipulated SAK. The time required for 50% platelet-rich clot lysis and the concentration required to obtain 50% clot lysis (C50) were reduced significantly across different concentrations of RGD-SAK comparing with SAK. Meanwhile, RGD-SAK was found to inhibit ADP-induced platelet aggregation in a concentration-dependent manner while SAK had negligible effect on platelet aggregation.
RGD-SAK possessed the bifunction to target platelet-rich clots and to block platelets aggregation, and thus may serve as a more potential thrombolytic agent with platelet-targeted thrombolytic and antiplatelet aggregation activities in compared with SAK.
Acute myocardial infarction is among the most prominent causes of death in the Western world. It is commonly caused by the formation of a pathologic clot that results in obstructing the blood flow to heart tissues. Staphylokinase (SAK), a 136-amino acid protein from certain lysogenic Staphylococcus aureus strains, is a plasminogen activator and a promising blood clot-dissolving agent with clinical potency that is at least as good as tPA [1, 2]. SAK does not bind directly to fibrin, it can bind indirectly through the fibrin binding of plasmin(ogen) by forming a 1:1 stoichiometric SAK-plasmin(ogen) complex. The resulting SAK-plasmin complex can then function as the plasminogen activator to convert plasminogen to plasmin for clot lysis . Although staphylokianse (SAK) is among the most promising blood dissolving agents, it is far from ideal. Recently, some studies have reported that, SAK could not mediate early reperfusion in 38% of treated patients and thrombolytic therapy employing staphylokinase is limited by rethrombosis of the very arteries being opened, which follows in a small but significant number of patients [1–3]. Development of more potent and faster-acting thrombolytic agents that can speed up the clot lysis process and have a lower rethrombosis rate would be desirable.
Now it is well known that platelets play a pivotal role in arterial thrombosis. Platelet-rich rather than fibrin-rich thrombosis was found to be responsible for many acute complications of angioplasty . Meanwhile, studies indicated that the reformed secondary clots are usually platelet-rich after thrombolytic therapy . Hence, it is interesting to hypothesize that the clot lysis efficacy of SAK can be enhanced by the application of the manipulated version of SAK that simultaneously targeted dissolve the platelet-rich clot and inhibit the reformation of blood clots by preventing platelets aggregation.
On activated platelets, the binding of surface glycoprotein GPIIb/IIIa to fibrinogen mediates platelet aggregation . GPIIb/IIIa receptor antagonist, such as Arg-Gly-Asp (RGD) peptide had been proved to have obvious antiplatelet activity in thrombus formation experiments [7, 8]. In order to improve the thrombolytic potential of SAK, as well as to introduce the antiplatelet activities, the mutant of SAK has been constructed. We substituted K35 with Arg to constitute a RGD motif, resulting in a novel SAK variant, designated as RGD-SAK, which was supposed to be recognized by the activated GPIIb/IIIa on the surface of platelet membrane. However, it is not very clear whether the engineered mutant obtain the hypothesized effects. In this study, this mutant RGD-SAK was constructed and over-expressed in E coli. Biochemical properties, platelet-targeted thrombolysis, and antiplatelet aggregation of the purified target protein were determined.
Construction, expression and purification of RGD-SAK
Physicochemical and biological characterization of purified RGD-SAK
To explore the structure of RGD-SAK, the purified sample was subjected to both N-terminal sequencing by the Edman degradation procedure and far-UV CD spectrum analysis. Sequencing of the first fifteen amino acid residues from the RGD-SAK (SSSFDKGKYKKGDDA) matched exactly with the mature SAK sequence. Analysis of the far-ultraviolet CD spectra (Fig. 2C) showed that SAK comprised 18.2% α-Helix, 36.7% β-Sheet, 45.0% turns and random coils while RGD-SAK comprised 19.3% α-Helix, 36.9% β-Sheet, 43.9% turns and random coils. These results revealed that the secondary structure of the two proteins were very similar and suggested that the substitution of K35with Arg did not change the secondary structure of SAK significantly.
The kinetics of equimolar mixture of human plasmin with RGD-SAK
0.39 ± 0.03
0.015 ± 0.002
0.033 ± 0.003
0.78 ± 0.05
0.031 ± 0.001
0.037 ± 0.005
Fibrin and platelet binding assay
Fibrin clot and platelet-rich clot lysis assay
The time required to lyse 50% of fibrin clot or Platelet-rich clot (T50%) and the concentration required to obtain 50% clot lysis (C50) with SAK or RGD-SAK
T50% of fibrin clot (min)
T50% of platelet-rich clot (min)
C50 of fibrin clot lysis (nM)
C50 of platelet-rich clot lysis (nM)
195 ± 8.7
198 ± 8.6
80 ± 8.5
200 ± 8.3
161 ± 9.3
166 ± 7.8
138 ± 8.5
123 ± 5.6
189 ± 9.9
93 ± 3.6
76 ± 8.3
38 ± 3.9
151 ± 6.3
59 ± 2.9
131 ± 9.7
33 ± 3.8
Antiplatelet aggregation assay
In this study, we report the construction and evaluation of a new class of bifunctional proteins consisting of two functional modules, RGD and SAK. To develop the novel SAK variant designated RGD-SAK with platelet-targeted thrombolytic and antiplatelet aggregation activity, K35 in SAK was substituted with Arg to constitute a RGD motif, which was testified to be recognized by the activated GPIIb/IIIa. As three-dimensional structures of SAK and its ternary complex with μPG (micro-plasminogen) have been resolved , various attempts to manipulate and optimize the structure and function of SAK have been reported [3–5]. One of them is to construct a targeted thrombolytic agent. In some research, the Arg-Gly-Asp (RGD) peptide was constructed in the SAK due to its affinity with GPIIb/IIIa on the membrane of activated platelets. However, the anticipated targeting effect to platelet-rich clots was either partially or completely lost in some studies, likely attributed to the positioning of RGD, which should not be blocked by unfavorable spatial structure or be cleaved off during the plasminogen activation process [10, 11]. By analysing the molecular modeling of the mutants, our previous study  suggested that loop 1 in SAK would be a rational introducing site for RGD. Research  found that the secondary structure sheet 1 (G22-D33) of SAK can be connected with sheet 2 (N37-I49) by loop 1, where the inserted RGD motif is supposed to be exposed on the solvent accessible surface of the SAK molecule and thus may allow access to the intergrin receptors. In addition, loop 1 is far from the active cleft in the SAK ternary complex , hence the mutation may have little effect on the SAK complex activity.
For biochemical and biological analysis of RGD-SAK, a convenient and effective method was developed to express and purify the target protein, which also provided an empirical basis for the establishment of standard operating procedure (SOP) for further large scale production. Compared with the conventional induction by IPTG (Isopropyl β-D-1-thiogalactopyranoside) , the temperature inducible expression under the control of pL and pR promoter in RGD-SAK expression is more feasible and cost-saving for fermentation. By optimizing fermentation condition, e.g. bacteria density and induction time, RGD-SAK was present in intracellular soluble state instead of inclusion body as reported previously , which is highly desirable for further purification. To obtain the highly purified and endotoxin-free protein, the cytosolic fractions after homogenization were subjected to cation chromatography and gel filtration to separate RGD-SAK, followed by anion chromatography to eliminate endoxin. The safety evaluation experiments testified that the highly purified, sterilized preparations contained low endotoxin levels, and were devoid of acute toxicity, which is safe and suitable for a more detailed clinical investigation.
Biochemical analysis indicated that RGD-SAK maintained the similar structure and the fibrinolytic function of SAK. The uniform N terminal sequence and similar CD spectrum suggested that these two molecules had similar structure. The measurement with fibrin plate and fibrin clot respectively revealed that the fibrinolytic activity of RGD-SAK was at least equal to that of SAK. The study further revealed that RGD-SAK can form a 1:1 RGD-SAK-plasmin complex which displayed an amidolytic activity comparable with that of the native SAK-plasmin binary complex. The similar catalytic efficiencies determined by kcat/Km of RGD-SAK in comparison with SAK revealed that the specific plasiminogen activating potential of the mutant remained almost intact. These above results indicated that the RGD existence in SAK would not disrupt the structure and the fibrinolytic function of SAK.
The platelet binding experiment indicated that RGD-SAK could bind the activated platelet in a dose dependent manner and with a higher affinity than SAK, which has weak ability to bind the platelet. In the platelet-rich clot lysis assay, the T50% values for RGD-SAK were significantly shortened. Analysis of these T50% values revealed a significant increase in platelet-rich clot lysis rate by RGD-SAK compared with SAK. Our data also demonstrated that RGD-SAK did not lead to enhanced fibrin clot lysis under in vitro conditions in comparison with SAK, which was correlated with their similar ability to bind the fibrin clots. By interpolation of the data of clot lysis assay, the C50 of the two proteins was similar in fibrin-rich clot while the C50 of RGD-SAK in platelet-rich clot was much shorter than equalmole SAK. These results suggested that RGD-SAK can target to the platelet-rich clots due to the direct selective binding to the activated platelets but not to fibrin which is also an important constituent of platelet-rich clots. Based on these results, we can conclude that RGD-SAK can accumulate on the platelet surface and thus enrich the thrombolytic activity at the site of the thrombus. Hence, it is possible that RGD-SAK would be directly targeted to thrombus in vivo because of their high affinities to platelets, which can therefore lyse the platelet-rich clots more efficiently than SAK due to the increased local concentration on the thrombus. In addition, it would be interesting to consider that the platelets themselves can suppress clot lysis by mechanical cross-linking, promotion of clot stability as well as release of fibrinolytic inhibitors . Some recent in vivo studies [17, 18] revealed that fibrinolysis can be accelerated by conjunctive use of anti-GP IIb/IIIa antibody. Hence, these findings imply that the RGD-SAK may accelerate lysis of platelet-rich clots via diverse mechanisms in vivo. Finally, it should be noted that a retarded renal clearance of RGD-SAK can also be expected due to its high affinities to the activated platelets and thus the resulting prolongated duration of action would allow use of low doses and/or less frequent administration of the agent. Therefore, if less RGD-SAK is required to achieve the same degree of clot lysis as SAK, the risk for side effects could also be minimized. We will further test the speculation by pharmacological and pharmacokinetic studies in vivo.
The use of RGD peptide as a platelet-targeted domain in the design of SAK mutant offers RGD-SAK another potential advantage of antiplatelet aggregation. It is well known that aggregation of blood platelets plays a pivotal role in the formation of thrombus and the RGD peptide is a component of ligands recognizing platelet intergrins [7, 8]. Therefore, the protein containing RGD has the potential ability to inhibit platelet aggregation and thus to be used in the prevention of arterial rethrombosis. In the antiplatelet aggregation assay in vitro, we have shown that the addition of the RGD sequence to SAK resulted in acquisition of the ability to prevent platelet aggregation. In the present study, the RGD-SAK had obvious inhibitory effects on different inducers (ADP or collagen or thrombin)-induced platelet aggregation in a dose dependent manner, while the SAK had almost no effect on platelet aggregation. Meanwhile, the efficiency of antiplatelet aggregation by RGD-SAK was comparable with that of RGD sequence alone. For ADP, collagen and thrombin are the most powerful physiological inducers of platelet aggregation after arterial injury and during rethrombosis, so the present data indicated that RGD-SAK possessed the ability to block platelet aggregation and hence should be more effective than SAK in preventing the formation of rethrmobosis after successful thrombolytic therapy, which would also be worthy of further research in vivo. Noticeably, for the thrombolysis and rethrombosis in vivo is a complex process, thrombin was also found to be closely related to this action. So, it's very interesting to speculate that the addition of antithrombin functional element to SAK may also accelerate the successful lysis of the thrombus as well as the protection against rethrombosis. Recently such a protein has been synthesized by Szemraj et al. , which cued in us that fusion antithrombin element with RGD-SAK may form a even more potent and faster thrombolytic agent with anti-rethrombosis properties than SAK. We will further manipulate and screen the rational SAK mutant on the purpose in the future.
In conclusion, the present study established the large scale expression and preparative purification method for RGD-SAK. The results of the in vitro study revealed that the purified mutant of SAK possessed the bifunction to target platelet-rich clots and to block platelets aggregation, and thus may be a more potent clot-dissolving agent for thrombolytic therapy compared with SAK.
Construction of E. coli expression vector of RGD-SAK
The DNA fragment encoding RGD-SAK was obtained by substituting K35 with Arg to constitute a RGD motif using PCR-based mutagenesis on the template of pSTE-SAK constructed in our previous research . in which gene encoding the mature region of SAK. The desired mutant was confirmed by DNA sequencing and cloned into expression vector pLY-4. Cytosolic expression was obtained by introducing the recombinant plasmids PLY-4-DGR into E coli JF1125 under the temperature inducible pL and pR promoter.
Large scale expression and preparative purification of RGD-SAK
Two-step fermentations were used in the protein expression. Fourteen-liter M9CA medium was inoculated with 1 L of an overnight bacteria culture in LB medium in a 20 L fermentor (Bioengineering, Swiss), and was grown at 30°C for 5 h. The culture was then transferred into a 300 L fermentor with 200 L M9CA medium, and grew at 30°C for another 5 h. The soluble expression of RGD-SAK was induced at 42°C for 2.5 h before harvesting. For RGD-SAK purification, the cell pellet collected by centrifugation was homogenized with a high pressure homogenizer (APV-2000, Denmark). The supernatant obtained was purified by Chromatography system (AKTA Explore, GE) at 4°C on a 12.5 × 40 cm column of SP-Sepharose (Amersham-Pharmacia Biotech, USA), equilibrated with 0.02 M NaAc-HAc (pH 5.6), and eluted with a linear salt gradient (0.01–1 M NaAc-HAc-NaCl). RGD-SAK containing fractions were further purified by 12.5 × 100 cm Sephadex G-50 (Amersham-Pharmacia Biotech, USA) and concentrated by application on a 7.5 × 50 cm column of Q-Sepharose (Amersham-Pharmacia Biotech, USA) with stepwise elution (0.01–1.0 M PB-NaCl). The RGD-SAK containing fractions were pooled and adjusted to 1 mg/ml, and the material was sterilized by filtration through a 0.22 μ m Millipore filter. Purified recombinant protein was analyzed by 15% SDS-PAGE. Rabbit polyclonal anti-SAK antibodies were used for the detection of RGD-SAK by western blot analysis.
Physicochemical characterization of RGD-SAK
The purity of this complex was confirmed by High-performance liquid chromatography (HPLC). The purified and concentrated samples were applied to a reversed phase C18 column (3.5 μ m, 2.1 × 100 mm) (Waters, Milford, MA, USA), using a 2690 Alliance HPLC (Waters, Milford, MA, USA). Samples were eluted with a 0.1% HCOOH (H2O): 0.1% HCOOH (CH3OH) gradient, 100:0 to 0:100 in 30 min at a flow rate of 0.2 ml/min. Protein mass spectrometry analysis was performed using Matrix-assisted Laser Desorption Ionization-Time of Flight (MALDITOF) Mass (Waters, Milford, MA, USA). Mass spectra of the samples were obtained using a micromass spectrometer equipped with electrospray ionization (ESI) source.
The structural analysis of the protein was performed using N-terminal sequencing and Circular dichroism spectroscopy (CD spectra). Briefly, Purified recombinant protein were transferred to a polyvinylidene difluoride (PVDF) membrane by electroblotting for N-terminal sequencing by automated Edman degradation procedure as described by Matsudaira . The obtained sequence was compared with the cDNA-deduced polypeptide sequence. The secondary structure of the protein was estimated from spectral simulations based on reference CD spectra of Yang et al .
Biological analysis of RGD-SAK
The methodology for evaluation of endotoxin contamination, bacterial sterility, and acute toxicity in mice of RGD-SAK was established according to Collen D et al. . Purified preparations of RGD-SAK were planted on agar plates, which were then incubated under aerobic and anaerobic conditions, on McConkie and chocolate-agar media, and in thioglycolate and tryptic soy broth media to test bacterial sterility. The limulus amoebocyte lysate assay was used to determine the endotoxin contamination of the preparations according to the instructions of the manufacturer (sFDA). The acute toxicity of RGD-SAK preparations versus saline was evaluated by intravenous bolus injection of 200 μ l of the purified sterilized material at a dose of 3 mg per mice in 6 mice every group (All animals care and animal experimentation involved in this work were in accordance with NIH guidelines and the approved procedures of the Institutional Animal Use and Care Committee at Fudan University.). The animals were observed for living state and acute reactions (tachypnea, restlessness, stupor) during an 8-day observation period.
Fibrinolytic activity assay on fibrin plate
Fibrinolytic activity of SAK and RGD-SAK was measured on fibrin plate . Petri dishes containing 0.5% agrose, 0.055 IU/ml thrombin, 5.5 μ g/ml plasminogen (PG) and 0.52 μ g/ml fibrinogen were prepared. On this solidified fibrin plate, wells of equal diameter were punched and 10 μ l/well of diluted appropriate concentration of SAK (1000 IU/ml, SFDA) or RGD-SAK was added and kept at 37°C for 16 h. The diameter of the halo around the well was measured to calculate the functional activity of SAK or RGD-SAK.
Activation of human plasminogen
The kinetics of plaminogen activation was determined according to Schlott B et al. . Plasminogen was incubated with equimolar SAK or RGD-SAK at 37°C for 1 h to generate the SAK-plasmin or RGD-SAK-plasmin bimolecular complex. The performed activator complex (final concentration 10 nM) was then mixed with different concentration of plasminogen (final concentration 0.625 μ M, 1.25 μ M, 2.5 μ M, 5 μ M, 10 μ M) and generation of plasmin was continuously monitored at 405 nm with S-2251 (3 mM). The kinetics constants were determined from Lineweaver-Burk plots.
Measurement of fibrin binding activity
Microtiter plate was coated overnight with cross-linked fibrin as described by Wu et al. . Briefly, Fibrinogen (5 μ g/ml) was added into the wells and left overnight at 4°C, then blocked with 2 mg/ml BSA for 2 h. The fibrinogen was incubated with PBS containing human thrombin (1 NIH unit/ml) and CaCl2 (20 mM) for 2 h at 37°C to form cross-linked state. The fibrin was partially digested with plasmin at 1 pmol/well for 10 min at the room temperature, then the microplate was washed with PBST to remove unbound plasmin. SAK or RGD-SAK in PBST containing 3% BSA was added into the wells at a final concentration of 200 nM for 2 h at the room temperature. After washing the plate with PBST for three times, unbound materials were removed. The SAK or RGD-SAK retained on the well was bound with polyclonal antibodies against SAK and HRP-conjugated anti-mouse secondary antibodies orderly. Substrate solution of TMB was added to develop the color and was stopped with 1 M H2SO4. The optical density was read at 450 nm for 30 min. As a blank, some wells were coated only with PBS but treated otherwise the same. The experiment was performed three times for both SAK and RGD-SAK.
Fibrin clot lysis assay
Ninety-six-well microtiter plates were used for clot lysis assay according to the method reported previously . The wells were loaded with thrombin (0.6 NIH unit/ml), human fibrinogen (1 mg/ml) and 20 mM CaCl2 in HBS buffer (10 mM HEPES, 130 mM NaCl, pH 7.4) and the polymerizing fibrin solution were incubated for 3 h at a room temperature. The clots formed were washed with HBS, and then layered with a solution containing 1.5 μ M hPlg and SAK, or RGD-SAK at varying concentrations (50, 100, 200 nM). After 30 min of incubation at room temperature, the surface of the clots was washed with HBS and the washed clots were layered with 1.5 μ M hPlg in HBS. The turbidity of the wells was measured as the optical density (OD) at 405 nm every 6 minutes, using a microtiter plate reader (Perkin Elmer 1420, VICTOR 3) until completion. T50% for clot lysis, which represented the time to achieve a 50% lysis of the fibrin clot, was calculated from the OD/time data. The concentration of fibrinolytic agent required to obtain 50% clot lysis in 3 hours (C50) was determined from plots of percent versus the concentration of test compound. Duplicate wells were prepared for each concentration of clot lysis of SAK or RGD-SAK in each experiment, and the experiment was repeated three times. As a control, some clots were layered only with HBS but treated otherwise the same.
Measurement of platelet binding activity
An ELISA method was used to assess the platelet binding activity of SAK and RGD-SAK . The activated human platelets induced by thrombin solution (0.2 IU/ml) were transferred to microtiter plate. SAK or RGD-SAK in the buffer A (50 mM Tris-HCl, 100 mM NaCl, 2 mM CaCl2, pH 7.4) was added to the wells at a final concentration of 25 nM, 50 nM, 100 nM, or 200 nM. After incubated in 37°C for 3 h, the plate was washed with PBST for three times to remove the unbound materials. SAK or RGD-SAK retained on the well was probed with polyclonal antibodies against SAK  followed by horseradish peroxidase (HRP)-conjugated anti-mouse secondary antibodies. The amount of HRP retained was assessed by using TMB (Tetrabenzidine) as HRP substrate. Color development was determined at 450 nm using a microplate reader (Perkin Elmer 1420, VICTOR 3). T50% for clot lysis, which represented the time to achieve a 50% lysis of the platelet-rich clot, was calculated from the OD/time data. The concentration of fibrinolytic agent required to obtain 50% clot lysis in 2 hours (C50) was determined from plots of percent versus the concentration of test compound. As a control, some wells were layered only with PBS but treated otherwise the same. The experiment was repeated three times.
Platelet-rich clot lysis assay
Platelet-rich clot was formed using the modified procedure described by zhang et al. . Clotting was initiated by mixing human thrombin (to 0.6 NIH unit/ml), human fibrinogen (1 mg/ml) and CaCl2 (to 20 mM) in human platelet. Each clot in the microtiter plate was layered with 100 μ l aliquot containing freshly mixed hPlg (1.5 μ M) and varied concentration of SAK or RGD-SAK (50, 100, 200 nM). After 30 min the surface of each clot was washed three times with HBS to remove unbound SAK or RGD-SAK and 100 μ l hPlg (1.5 μ M) were layered on each clot. The clot lysis process was monitored by measuring the turbidity using a microtiter plate reader. Duplicate wells were taken in each one of the three repeated experiments. As a control, some clots were layered only with HBS but treated otherwise the same.
Inhibition of platelet aggregation
Platelet aggregation assay was performed with a modified method described by Seymour et al. . Isolated Platelet-rich plasma (PRP) was incubated for 15 min at 37°C with either SAK (diluted by saline, 2.5, 5, 10, 20, 40 μM) or equimolar concentrations of RGD-SAK or RGD (Sigma, USA) prior to the platelet aggregation stimulation with ADP (10 μM) or collagen (1 μg/ml) or thrombin 1 U/ml. The aggregation response was recorded using a dual-channel aggregometer (APACT Labor, Germany) and aggregation results were expressed as percent inhibition of platelet aggregation. Saline was choosed to be control.
Data are expressed as means ± SD. One-way analysis of variance (ANOVA) or student's t test was used where applicable. The differences with P-values < 0.05 were considered statistically significant.
We thank Prof. Xinyuan Liu (Shanghai Institute of Biochemistry, Chinese Academic of Sciences) for kindly providing the expression vector pLY-4 and E. Coli JF1125. This wok was supported by National 863 high-technology program (103-13-01-02, 2001) and the key Project from the shanghai Science and Technology Committee of China (No. 05DZ19303).
- Collen D, Van de Werf F: Coronary thrombolysis with recombinant staphylokinase in patients with evolving myocardial infarction. Circulation 1993,87(6):1850-1853.View ArticlePubMedGoogle Scholar
- Vanderschueren S, Stockx L, Wilms G, Lacroix H, Verhaeghe R, Vermylen J, Collen D: Thrombolytic therapy of peripheral arterial occlusion with recombinant staphylokinase. Circulation 1995,92(8):2050-2056.View ArticlePubMedGoogle Scholar
- Armstrong PW, Burton J, Pakola S, Molhoek PG, Betriu A, Tendera M, Bode C, Adgey AA, Bar F, Vahanian A, Van de Werf F, CAPTORS II Investigators: Collaborative Angiographic Patency Trial Of Recombinant Staphylokinase (CAPTORS II). Am Heart J 2003,146(3):484-488. 10.1016/S0002-8703(03)00312-0.View ArticlePubMedGoogle Scholar
- Mazur W, Kaluza G, Kleiman NS: Antiplatelet therapy for treatment of acute coronary syndromes. Cardiol Clin 1999,17(2):345-357. 10.1016/S0733-8651(05)70079-5.View ArticlePubMedGoogle Scholar
- Yasuda T, Gold HK, Yaoita H, Leinbach RC, Guerrero JL, Jang IK, Holt R, Fallon JT, Collen D: Comparative effects of aspirin, a synthetic thrombin inhibitor and a monoclonal antiplatelet glycoprotein IIb/IIIa antibody on coronary artery reperfusion, reocclusion and bleeding with recombinant tissue-type plasminogen activator in a canine preparation. J Am Coll Cardiol 1990,16(3):714-722.View ArticlePubMedGoogle Scholar
- Ruoslahti E: RGD and other recognition sequences for integrins. Annu Rev Cell Dev Biol 1996, 12: 697-715. 10.1146/annurev.cellbio.12.1.697.View ArticlePubMedGoogle Scholar
- Yamada T, Shimada Y, Kikuchi M: Integrin-specific tissue-type plasminogen activator engineered by introduction of the Arg-Gly-Asp sequence. Biochem Biophys Res Commun 1996,228(2):306-311. 10.1006/bbrc.1996.1657.View ArticlePubMedGoogle Scholar
- Ruoslahti E, Pierschoacher MD: New perspectives in cell adhesion: RGD and integrins. Science 1987,238(4286):491-497. 10.1126/science.2821619.View ArticlePubMedGoogle Scholar
- Parry MA, Fernandez-Catalan C, Bergner A, Huber R, Hopfner KP, Schlott B, Guhrs KH: The ternary microplasmin-staphylokinase-microplasmin complex is a proteinase-cofactor-substrate complex in action. Nat Struct Biol 1998,5(10):917-923. 10.1038/2359.View ArticlePubMedGoogle Scholar
- Wu SC, Castellino FJ, Wong SL: A fast-acting, modular-structured staphylokinase fusion with kringle-1 from human plasminogen as the fibrin-targeting domain offers improved clot lysis efficacy. J Biol Chem 2003,278(20):18199-18206. 10.1074/jbc.M210919200.View ArticlePubMedGoogle Scholar
- Van Zyl WB, Pretorius GH, Lamprecht S, Roodt JP, Kotze HF: PLATSAK, a potent antithrombotic and fibrinolytic protein, inhibits arterial and venous thrombosis in a baboon model. Thromb Res 2000,98(5):435-433. 10.1016/S0049-3848(00)00204-8.View ArticlePubMedGoogle Scholar
- Su HB, Zhang YG, He JT, Mo W, Zhang YL, Tao XM, Song HY: Construction and characterization of novel staphylokinase variants with antiplatelet aggregation activity and reduced immunogenecity. Acta Biochim Biophys Sin 2004,36(5):336-342.View ArticlePubMedGoogle Scholar
- Rabijns A, de Bondt HL, de Ranter C: Three-dimensional structure of staphylokinase, a plasminogen activator with therapeutic potential. Nat Struct Biol 1997,4(5):357-360. 10.1038/nsb0597-357.View ArticlePubMedGoogle Scholar
- Schlott B, Hartmann M, Guhrs KH, Birch-Hirschfeid E, Pohl HD, Vanderschueren S, Van de Werf F, Michoel A, Collen D, Behnke D: High yield production and purification of recombinant staphylokinase for thrombolytic therapy. Biotechnology (N Y) 1994,12(2):185-189. 10.1038/nbt0294-185.View ArticleGoogle Scholar
- Cheng A, Song G, Su HB, Yu M, Li YY, Song HY: The renaturation and purification of RGD-staphylokinase by gel filtration. Sheng Wu Gong Cheng Xue Bao 2002,18(6):693-697.PubMedGoogle Scholar
- Konstantinides S, Schafer K, Thinnes T, Loskutoff DJ: Plasminogen activator inhibitor-1 and its cofactor vitronectin stabilize arterial thrombi after vascular injury in mice. Circulation 2001,103(4):576-583.View ArticlePubMedGoogle Scholar
- Collet JP, Montalescot G, Lesty C, Soria J, Mishal Z, Thomas D, Soria C: Disaggregation of in vitro preformed platelet-rich clots by abciximab increases fibrin exposure and promotes fibrinolysis. Arterioscler Thromb Vasc Biol 2001,21(1):142-148.View ArticlePubMedGoogle Scholar
- Huang TC, Jordan RE, Hantgan RR, Alevriadou BR: Differential effects of c7E3 Fab on thrombus formation and rt-PA-mediated thrombolysis under flow conditions. Thromb Res 2001,102(5):411-425. 10.1016/S0049-3848(01)00260-2.View ArticlePubMedGoogle Scholar
- Szemraj J, Walkwoiak B, Kawecka I, Janiszewska G, Buczko W, Bartkowiak J, Chabielska E: A new recombinant thrombolytic and antithrombotic agent with higher fibrin affinity – a staphylokinase variant. I. In vitro study. J Thromb Haemost 2005,3(10):2156-2165. 10.1111/j.1538-7836.2005.01480.x.View ArticlePubMedGoogle Scholar
- Matsudaira P: Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes. J Biol Chem 1987,262(21):10035-10038.PubMedGoogle Scholar
- Yang YW, Teng CC: Circular dichroism and fluorescence studies of polyomavirus major capsid protein VP1. J Protein Chem 1998,17(1):61-71. 10.1023/A:1022542631609.View ArticlePubMedGoogle Scholar
- Collen D, Moreau H, Stockx L, Vanderschueren S: Recombinant staphylokinase variants with altered immunoreactivity. II: Thrombolytic properties and antibody induction. Circulation 1996,94(2):207-216.View ArticlePubMedGoogle Scholar
- Bi Q, Cen X, Huang Y, Zhu S: Construction and characterization of trifunctional single-chain urokinase-type plasminogen activators. Eur J Biochem 2002,269(6):1708-1713. 10.1046/j.1432-1327.2002.02816.x.View ArticlePubMedGoogle Scholar
- Schlott B, Gührs KH, Hartmann M, Röcker A, Collen D: Staphylokinase requires NH2-terminal proteolysis for plasminogen activation. J Biol Chem 1997,272(9):6067-6072. 10.1074/jbc.272.9.6067.View ArticlePubMedGoogle Scholar
- Wu SC, Yeung JC, Duan Y, Ye R, Szarka SJ, Habibi HR, Wong SL: Functional production and characterization of a fibrin-specific single-chain antibody fragment from Bacillus subtilis: effects of molecular chaperones and a wall-bound protease on antibody fragment production. Appl Environ Microbiol 2002,68(7):3261-3269. 10.1128/AEM.68.7.3261-3269.2002.PubMed CentralView ArticlePubMedGoogle Scholar
- Zhang L, Wang J, Yu M, Ru B: Functional properties of a recombinant chimeric plasminogen activator with platelet-targeted fibrinolytic and anticoagulant potential. Mol Genet Metab 2004,82(4):304-311. 10.1016/j.ymgme.2004.05.008.View ArticlePubMedGoogle Scholar
- Ye R, Kim JH, Kim BG, Szarka S, Sihota E, Wong SL: High-level secretory production of intact, biologically active staphylokinase from Bacillus subtilis. Biotechnol Bioeng 1999,62(1):87-96. 10.1002/(SICI)1097-0290(19990105)62:1<87::AID-BIT10>3.0.CO;2-I.View ArticlePubMedGoogle Scholar
- Seymour JL, Henzel WJ, Nevins B, Stults JT, Lazarus RA: Decorsin. A potent glycoprotein IIb-IIIa antagonist and platelet aggregation inhibitor from the leech Macrobdella decora. J Biol Chem 1990,265(17):10143-10147.PubMedGoogle Scholar
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