Chromosomal duplications and cointegrates generated by the bacteriophage lamdba Red system in Escherichia coli K-12
© Poteete et al; licensee BioMed Central Ltd. 2004
Received: 20 September 2004
Accepted: 13 December 2004
Published: 13 December 2004
An Escherichia coli strain in which RecBCD has been genetically replaced by the bacteriophage λ Red system engages in efficient recombination between its chromosome and linear double-stranded DNA species sharing sequences with the chromosome. Previous studies of this experimental system have focused on a gene replacement-type event, in which a 3.5 kbp dsDNA consisting of the cat gene and flanking lac operon sequences recombines with the E. coli chromosome to generate a chloramphenicol-resistant Lac- recombinant. The dsDNA was delivered into the cell as part of the chromosome of a non-replicating λ vector, from which it was released by the action of a restriction endonuclease in the infected cell. This study characterizes the genetic requirements and outcomes of a variety of additional Red-promoted homologous recombination events producing Lac+ recombinants.
A number of observations concerning recombination events between the chromosome and linear DNAs were made: (1) Formation of Lac+ and Lac- recombinants depended upon the same recombination functions. (2) High multiplicity and high chromosome copy number favored Lac+ recombinant formation. (3) The Lac+ recombinants were unstable, segregating Lac- progeny. (4) A tetracycline-resistance marker in a site of the phage chromosome distant from cat was not frequently co-inherited with cat. (5) Recombination between phage sequences in the linear DNA and cryptic prophages in the chromosome was responsible for most of the observed Lac+ recombinants. In addition, observations were made concerning recombination events between the chromosome and circular DNAs: (6) Formation of recombinants depended upon both RecA and, to a lesser extent, Red. (7) The linked tetracycline-resistance marker was frequently co-inherited in this case.
The Lac+ recombinants arise from events in which homologous recombination between the incoming linear DNA and both lac and cryptic prophage sequences in the chromosome generates a partial duplication of the bacterial chromosome. When the incoming DNA species is circular rather than linear, cointegrates are the most frequent type of recombinant.
The Red recombination system of bacteriophage λ promotes efficient double strand break repair/recombination. An Escherichia coli strain in which RecBCD has been genetically replaced by Red exhibits greatly elevated levels of recombination between its chromosome and short linear double-stranded DNA species sharing sequences with the chromosome .
Results and discussion
Genetic requirements for Lac+ recombinant formation
Genetic requirements of Lac+ recombinant formation
1.00 ± 0.16
0.01 ± 0.01
0.05 ± 0.02
0.34 ± 0.06
0.22 ± 0.08
1.00 ± 0.31
0.51 ± 0.01
0.12 ± 0.09
0.06 ± 0.00
0.03 ± 0.02
1.00 ± 0.13
0.01 ± 0.01
0.05 ± 0.03
0.34 ± 0.25
0.08 ± 0.02
1.00 ± 0.14
0.29 ± 0.12
0.03 ± 0.03
0.01 ± 0.01
0.02 ± 0.01
The reason for considering mechanisms other than homologous recombination in the formation of the Lac+ recombinants is because of the expectation that some of the phage-borne lac::cat sequences would be attached to phage sequences. Heitman et al.  showed that double strand breaks generated by a restriction endonuclease in vivo are rapidly repaired by DNA ligase. The results of previous physical studies with other substituted λ phages cut in vivo by PaeR7 lead to the expectation that the λ lac::cat819 chromosome would be uncut, or only singly cut, much of the time in the infected cell [2, 6].
Preliminary characterization of the Lac+ recombinants formed in cells which were wild-type for all the recombination functions revealed that most, possibly all, were unstable. When streaked on plates containing chloramphenicol and X-Gal, they segregated Lac- (colorless) progeny at variable frequencies. Southern gel analysis of chromosomal DNA from unstable Lac+ recombinants revealed that some of them were multiploid for lacZ (not shown). When the lac::cat dsDNA was delivered into the cell by a λ vector bearing a tetracycline resistance determinant (Δ nin::tet859 – described below) neither the Lac+ nor Lac- recombinants acquired tetracycline resistance. These findings suggest that the process which formed the recombinants did not involve the entire phage chromosome.
A baseline level of Lac+ recombinants was to be expected in these crosses. The recombination event pictured in Figure 1A, occurring in a cell with a pre-existing duplication of the lac locus, will produce a Lac+, chloramphenicol-resistant recombinant. Data presented below, in which simpler lac::cat recombining substrates generate Lac+ recombinants at a frequency approximately 1000-fold lower than Lac-, suggest the frequency of spontaneous lac duplications in Red+ but otherwise wild type E. coli is approximately 10-3, consistent with estimates of spontaneous duplication frequency in Salmonella made by Roth et al. . The Lac+ recombinants formed in the crosses summarized in Table 1 occurred at a much higher frequency – approaching 5% of the infected cells – suggesting that pre-existing chromosomal duplications were not involved in the generation of the majority of the Lac+ recombinants. Apparently, normal Red-mediated homologous recombination between the phage and bacterial chromosomes duplicated or amplified sequences in the bacterial chromosome.
The crosses described above were done by infecting log phase host cells grown in rich medium. Such cells contain multiple copies of their chromosome. Complications might arise if the lac::cat segment were to recombine with more than one chromosome at a time. Further complicating interpretation of the experiment, the cells were infected at a multiplicity of 10 phages per cell.
Recombinant formation by λ lac::cat variants
cut both sides
0.54 ± 0.06
0.92 ± 0.04
2.8 ± 0.07
cut neither side
0.055 ± 0.001
cut neither side
0.039 ± 0.002
cut both sides
0.93 ± 0.08
3.7 ± 0.04
4.6 ± 0.2
cut neither side
0.10 ± 0.03
λ lac::cat819 nintet
0.28 ± 0.04
λ lac::cat819 nintet
0.024 ± 0.003
λ lac::cat819 nintet
0.027 ± 0.005
λ lac::cat819 nintet
0.001 ± 0.002
λ lac::cat819 nintet
0.008 ± 0.008
λ cat988 nintet
right flank only
0.008 ± 0.003
λ cat989 nintet
left flank only
0.003 ± 0.0002
λ cat995 nintet
0.004 ± 0.0002
0.002 ± 0.001
0.002 ± 0.0002
Recombinant formation by single-cut variants of λ lac::cat
To examine the dependence of Lac- and Lac+ recombinant formation on the structure of the DNA substrate, we constructed variants of λ lac::cat, which are diagrammed in Figure 1. λ lac::cat819, the progenitor of this series, has two PaeR7 sites. Upon infecting a host cell bearing the Δ recBCD::Ptac-gam-bet-exo-pae-cI substitution, it injects its chromosome, which circularizes. Expression of its lytic genes, including those necessary for phage DNA replication, is blocked by the action of cI repressor. Cutting of its chromosome by the PaeR7 restriction endonuclease in the infected cell releases a 3.5 kbp linear dsDNA, consisting of the cat gene and 1.3 kbp flanks of lac sequences on the right and left. (In this and subsequent descriptions, "right" refers to the upstream, or 5' end of the lac operon). The ends of the flanking lac sequences match precisely their counterparts in the chromosome.
λ lac::cat930 and λ lac::cat931 have only single PaeR7 sites, on the right and left, respectively. Cutting by PaeR7 in the infected cell should produce large linear dsDNAs related to the lac::cat819 fragment, but with long tails of non-homologous DNA extending from the left and right sides, respectively. Interestingly, both of these single-cut phages produce recombinants more efficiently than the double-cut λ lac::cat819, in both recG+ and Δ recG backgrounds (Table 2). The right-cut λ lac::cat930 produces relatively more Lac+ recombinants, whereas the left-cut λ lac::cat931 produces fewer. The frequency of Lac+ λ lac::cat931 recombinants, 0.2%, is roughly consistent with the expected frequency of pre-existing lac duplications in the population of infected cells, suggesting that λ lac::cat931 does not generate duplications, but λ lac::cat930 does. We cannot rule out the possibility that some of the 0.2% Lac+ recombinants made by λ lac::cat931 are cointegrates. Cointegrate formation is discussed further in the next section.
Recombinant formation by circular λ lac::cat
λ lac::cat929 has no PaeR7 site. Its chromosome should stay circular in the infected Δ recBCD::Ptac-gam-bet-exo-pae-cI cell. Surprisingly, it produces chloramphenicol-resistant recombinants only 10-fold less efficiently than λ lac::cat819. Unlike the PaeR7-cuttable λ lac::cat variants, λ lac::cat929 also efficiently produces weakly chloramphenicol-resistant microcolonies (not counted in the data presented in Table 2). The provenance of the microcolonies is readily understandable. The uncuttable λ lac::cat929 chromosome cannot replicate; it also is not destroyed, and should produce chloramphenicol acetyl transferase in the cell in which it resides. The infected cell, and perhaps its descendants for one or two generations, might be able to divide in the presence of low-concentration chloramphenicol.
Approximately half of the strongly chloramphenicol-resistant recombinants generated by the uncuttable phage λ lac::cat929 are Lac- (Table 2). This observation suggests that they are "legitimate" recombinants, in which the single chromosomal copy of lacZ is replaced by lacZ::cat. These recombinants were unexpected. All Red-mediated recombination events are thought to involve at least one linear partner . How, then, can circular λ lac::cat929 recombine with the circular E. coli chromosome? Three possible explanations were considered. (1) Some of the λ lac::cat929 chromosomes might be cut by the EcoK restriction endonuclease. The infected cells contained a functional EcoK restriction-modification system. λ lac::cat929 was grown in a similarly EcoK+ host, and therefore was presumably EcoK-modified. However, if the modification were not complete, there might be residual EcoK restriction activity, resulting in 10% of the phage chromosomes being cut. (The phage chromosome contains five EcoK sites. If each site were 98% methylated, then approximately one in ten phage chromosomes would have a single unmethylated site). (2) The production of the recombinants might not be Red-mediated. (3) The linear partner might be a broken bacterial chromosome.
To test the first explanation, we replaced the gene encoding the EcoK restriction endonuclease, hsdR, with a tetracycline resistance determinant. This replacement had little effect on the formation of recombinants by λ lac::cat929 (Table 2).
To explore further the origins of the recombinants formed between circular phage chromosomes and the bacterial chromosome, we constructed a series of bacterial strains bearing variants of the Δ recBCD::Ptac-gam-bet-exo-pae-cI substitution lacking pae. In these hosts, no cutting takes place at PaeR7 sites, and so all of the lac::cat-substituted phages should remain circular. The results of crosses in these strains are shown in Table 2. As in the Pae+ crosses, elimination of hsdR in the Pae- background did not reduce the yield of recombinants. In the Pae- background, elimination of recA reduced the yield of recombinants twenty-fold. The great majority of the residual recombinants were Lac+, suggesting that their formation was by a process not necessarily involving homologous recombination. Elimination of red reduced the yield of recombinants three-fold, showing that most, but not all of the recombination events were Red-mediated. As in the Red+ cross, approximately half of the recombinants were Lac-, indicating that formation of both Lac- and Lac+ recombinants was Red-mediated in approximately the same proportions. Thus, the second hypothesis mentioned above, that the recombinants are formed by a Red-independent process, is not supported. The third hypothesis, that the linear partner might be a broken bacterial chromosome, is thus favored. It additionally seems reasonable, given that spontaneous double-strand breaks are frequent in E. coli .
We sought to detemine what proportion of the circular phage-by-chromosome recombinants were cointegrates, by testing for co-inheritance of cat and a tetracycline resistance-conferring element present at a remote location in the phage chromosome. Among the Lac+ recombinants produced in the Pae+ host TP507 (Table 2), only 2 out of 39 tested were found to be tetracycline-resistant; none of the 40 Lac- recombinants we tested was tetracycline-resistant. This result was consistent with our earlier finding that the great majority of recombinants formed in the high mulitiplicity infections of log phase cells acquired only the homology-flanked cat segment of the infecting λ chromosome. In contrast, in the Pae- host, 27 of 55 Lac- recombinants, and 23 of 25 Lac+ recombinants, were additionally tetracycline-resistant. These observations indicate that the most frequent Red-generated product of recombination between the uncut phage chromosome and the bacterial chromosome is a cointegrate.
The ability of the λ Red system to promote recombination events involving short sequence homologies makes it particularly useful for genetic engineering. Yu et al.  reported that a linear cat cassette with 1000 bp homologous flanks was only 10-fold more efficient than one with 40 bp flanks. Their experiments were done with cells which had been subjected to heat shock and electroporation. To test the efficiency of short-flank recombination under less extreme conditions, we constructed a short-flank λ lac::cat variant.
The fragment released by PaeR7 from the chromosome of λ lac::cat921 consists of the cat gene flanked by 40 bp sequences corresponding exactly to the terminal 40 bp at each end of the 1.3 kbp flanks of λ lac::cat819. As shown in Table 2, λ lac::cat921 exhibits a several hundred-fold lower efficiency of recombinant formation than its long-flank counterpart, in both recG+ and Δ recG backgrounds. λ lac::cat921 was similarly inefficient in log phase cells (data not shown). These observations suggest that short-flank recombination may not be a significant activity of the Red system in nature. However, they do not speak to the question of whether long flanks work better because they provide a larger homology target for synapsis, or because they provide non sequence-specific protection, perhaps delaying exonucleolytic degradation of the recombining sequences long enough to permit recombination to take place.
Recombination with inverted partners
Bacterial strains used in this study
Source, reference, or construction
Δ(recC-ptr-recB-recD)::P lac -gam-bet-exo-kan
Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-cat
Poteete et al., 1999
Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822
Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 ruvC53 eda::Tn 10
Poteete and Fenton, 2000
Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 Δ(srl-recA)306::Tn10
Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 recG258::kan Δ(srl-recA)306::Tn 10
Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 Δ ruvAB6203::tet
Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 Δ recG6202
Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 Δ recG6202 ruvC53 eda::Tn10
Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 Δ recG6202 Δ ruvAB6203::tet
Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 Δ sulA6209::tet
Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 Δ recG6202 Δ sulA6209::tet
Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 Δ sulA6209::tet lexA71::Tn5
Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 Δ recG6202 Δ sulA6209::tet lexA71::Tn5
Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 Δ sulA6209::tet recO1504::Tn5
Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 Δ sulA6209::tet recF400::Tn5
Δ (recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 Δ sulA6209::tet recR252::Tn10–9kan
Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 Δ recG6202 Δ sulA6209::tet recO1504::Tn5
Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 Δ recG6202 Δ sulA6209::tet recR252::Tn10–9kan
Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 Δ recG6202 Δ sulA6209::tet recF400::Tn5
Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 Δ recQ6216::tet
Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 Δ recG6202 Δ recQ6216::tet
Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 Δ hsdR::tet
TP507 × PCR productb
Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-cI978 Δ hsdR::tet
TP849 × P1(TP839)
Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-cI978
KM22 × pTP978 linearb
Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-cI978 Δ recA6207::tet
TP849 × P1(TP796)
Δ(recC-ptr-recB-recD)::P tac -gam-cI996
KM32 × pTP996 linearb
Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-cat
MG1655 × P1(KM32)
Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822
TP798 × pTP822 linearb
Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-bla979
TP798 × pTP979 linearb
Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-bla979 Δ lac::cat
TP832 × PCR productb
Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 Δ lac::cat
TP829 × P1(TP872)
Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 lac-inv
TP890 × pTP1034 linearb
Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-bla979 lac-inv
TP872 × pTP1034 linearb
Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822
Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 Δ recA6207::tet
The single-cut lac::cat930 and lac::cat931 alleles were constructed by replacing the left-side and right-side PaeR7 sites, respectively, of pTP819, with XbaI sites. To produce inverted derivatives of these two alleles, the plasmids pTP930 and pTP931 were digested with XbaI and XhoI (a PaeR7 isoschizomer); lac::cat inserts and bla-ori backbone fragments from the two plasmids were exchanged. The inverted alleles were then crossed into phage λ, as described in the Methods section.
In constructing the chromosomal inversion, we switched genetic backgrounds, from the AB1157-derived strains with which most recombination studies have been done, to MG1655, the sequenced wild type E. coli K-12 . In the MG1655 background, the same directionality of Lac+ recombinant formation was observed. The efficiencies of recombination of the MG1655 derivatives with both λ lac::cat930 and λ lac::cat931, and the efficiency of Lac+ recombinant formation by λ lac::cat930, were slightly elevated relative to those in the corresponding AB1157-derived strain.
Recombination with electroporated linear DNA species
Recombinant formation by variant linear DNA species
Recombining linear species
Bam + Xho
27 ± 2
Bam + Xho + Kpn
0.6 ± 0.2
Bam + Xho
27 ± 2
Bam + Xho
14 ± 0.1
Sph + Xho
95 ± 3
BsrG + Xho
99 ± 0.5
Bam + Xho
96 ± 2
Bam + Xho
0.2 ± 0.04
The hypothesis that the high-frequency Lac+ chloramphenicol-resistant recombinants are generated by homologous recombination with cryptic prophage sequences in the chromosome predicts that such recombinants would not be generated in an E. coli strain in which the cryptic prophages are deleted. To test this prediction, we electroporated linear lac::cat930 DNA into TP750, a Red+ derivative of MDS12, the reduced-genome E. coli strain constructed by Kolisnychenko et al. . In this cross, Lac+ recombinants constituted only 0.06% (average of 6 measurements) of the total chloramphenicol-resistant progeny, a frequency no higher than expected for spontaneous pre-existing duplications in the bacterial chromosome.
We constructed several other linear DNA substrates to test the generality of the model in Figure 6. The abilities of these substrates to generate Lac+ recombinants are shown in Table 3. The first of these was a derivative of lac::cat930 lacking its χ site. The χ site is located in the right lac flank, which is labeled "B" in Figures 3–6, close to the cat gene. The orientation of this χ site is such that it would be expected to interact productively with RecBCD enzyme approaching from the PaeR7- or Xho-generated right end of lac::cat930. While no activity of χ in these crosses was expected, as the bacteria lack RecBCD, the directionality of Lac+ recombinant formation was reminiscent of the directionality of χ-RecBCD interaction (see  for a review). The Δχ substrate was just as active as the χ + version, showing that χ does not contribute significantly to Lac+ recombinant formation. Similarly, a lac::cat930 derivative (pTP1052) lacking its sequences derived from phage P22 was equally proficient at generating Lac+ recombinants. A derivative missing the left lac flank made Lac+ recombinants almost exclusively; the small number of Lac- recombinants in this case probably represent cointegrates made by uncut plasmid DNA in the fragment preparation.
Structures of the λ lac::cat Lac+ recombinants
The major class of Lac+ chloramphenicol-resistant recombinants formed in crosses involving infection by λ lac::cat819 (cut left and right) and λ lac::cat930 (cut right) behave like their counterparts generated by electroporation of linear dsDNAs into Red+ cells: they segregate Lac- chloramphenicol-resistant clones at low frequency, and Lac+ chloramphenicol-sensitives at high frequency (data not shown). In addition, their formation also depends upon the presence of cryptic prophages in the chromosome: λ lac::cat819 was found to produce Lac+ chloramphenicol-resistant recombinants as only 0.13% (average of six measurements) of the total chloramphenicol-resistant progeny in crosses with TP750. These properties suggested both kinds of recombinant might have the same structures as well, but PCR tests of 50 of the phage-generated recombinants with the primers used to demonstrate type I, II, and III recombinants (Figure 8) were negative (data not shown).
Further computer analysis revealed a cryptic prophage sequence which could recombine with the phages, but not with the shorter, plasmid-derived linear DNAs, producing recombinants by the mechanism drawn in Figure 6: a 3.5 kbp patch of DLP12 closely matching sequences in the vicinity of the λ cos site. This site is located immediately to the left of the tfaD locus in the chromosome. Recombinants formed by crossing over at this site and at the right lac flank would be expected to contain duplications of bacterial sequences identical to those of type I recombinants (pictured in Figure 6), but to contain a larger part of the phage chromosome as well. PCR tests of six λ lac::cat819 and six λ lac::cat930 recombinants with primers designed to demonstrate cos recombinant junctions showed that all twelve had them. An example is shown in Figure 8.
The predominance of cos recombinants over tfa recombinants among the phage-generated Lac+ recombinants is to be expected. First, λ Exo, traveling in from the end of the long left-side non-homologous tail of λ lac::cat930 encounters cos before tfa; cos therefore presumably has a kinetic advantage. Second, the bacterial cos homology patch is significantly larger than the tfa homologies. A third possible advantage of cos is that, at least in some parts of the phage lytic cycle, it is the site of a double-strand break for the DNA encapsulation step of phage assembly. We expected that cos would be unbroken almost all the time in our crosses. The λ chromosome is linear at the time of injection, but is rapidly circularized by annealing and ligation . The other parts of the phage lytic cycle are inhibited by the presence of cI repressor in the infected cells. Even so, the hypothesis that Red sometimes manages to gain access to cos ends in these crosses remains plausible; particularly so because Red is present in the cell at all times, whereas, in a normal infection, Red is not present until its genes are expressed from the phage chromosome. An unexpectedly significant presence of cos ends in the infected cells could also help explain the otherwise surprisingly high frequency of chloramphenicol-resistant recombinants seen in crosses involving phage chromosomes not cut by restriction endonucleases, described above.
One aspect of Lac+ recombinant formation by λ lac::cat819 is not explained by the model of Figure 6. The λ lac::cat819 contains two PaeR7 sites. Cutting by PaeR7 should release a linear dsDNA with lac flanks and no attached non-lac DNA. This DNA species did not generate Lac+ recombinants (above the background of pre-existing duplications) when electroporated directly into cells (Table 3). How does it apparently do so in the infected cells? As discussed above, it is expected that the λ lac::cat819 chromosome would be uncut, or only singly cut, much of the time in the infected cell. If it recombined at a time at which it was cut only on the right, the type of recombinant pictured in Figure 6 could be formed. In this recombinant, an uncut and unmodified PaeR7 site would sit in the chromosome. Presumably, the presence of this site would make the recombinant unstable, initially; but the PaeR7 site might eventually become modified, that is, escape restriction. The pae-expressing strains employed in these experiments restrict plaque formation by single PaeR7 site-bearing λ phages only approximately 5-fold (unpublished data).
The bacteriophage λ Red recombination system is of general interest for two main reasons. First, it is an intensively characterized and relatively simple system, which serves as a model for studies of homologous recombination [15–17]. Second, it has emerged as a powerful tool for genetic engineering in gram-negative enteric bacteria [1, 9, 18–24]. It was therefore of interest to elucidate the structures of the previously observed but unexplained Lac+ recombinants formed by recombination between lac::cat-bearing λ phages and the bacterial chromosome, as well as the mechanism of their formation. Our studies uncovered a diversity of recombinant structures, including complex duplications and cointegrates. Recombination between λ and cryptic prophage sequences in the bacterial chromosome was found to be the most significant mechanism generating Lac+ recombinants. All of the Lac+ recombinants could be generated by homologous recombination events of types which have been previously described. In particular, there was no evidence for end-joining or other non-homologous recombination events. Perhaps the most surprising finding was the high frequency with which large duplications in the bacterial chromosome – up to 1 Mbp – could be generated by the Red system.
E. coli strain DH5α (λ pir)  was used for propagation of plasmids bearing the R6K origin. Other strains used in this study are described in Table 4. The Δ hsdR::tet allele in TP842 was constructed by using a Tn 10-containing E. coli strain as template in PCR with primers hsdRut (5'-TTGGACAGGCCCGCACAGCAATGGATTAATAACAATGATGCTCGACATCTTGGTTACCGT-3') and hsdRdt (5'-GCTGAATTTGCCCAGCAGGGTATCGAGATTATCGTCAAAGCGCGGAATAACATCATTTGG-3'). The Δ lac::cat allele in TP872 was constructed by using a Tn 9-containing E. coli strain as template in PCR with primers cat15 (5'-TCTGGTGGCCGGAAGGCGAAGCGGCATGCATTTACGTTGAATGAGACGTTGATCGGCACG-3') and cat16 (5'-AGAGTACATCTCGCCGTTTTTTCTCAATTCATGGTGTACAATTCAGGCGTAGCACCAGGC-3').
An EcoR1 fragment of λ cI857 Sam7 containing the nin region genes was cloned into the EcoR1 site of pBR322. In the resulting plasmid, pnin, the nin genes are read clockwise in the conventional map of pBR322. pTP772 was constructed by deleting sequences between the two HindIII sites of pnin. pTP859 was constructed by cutting pTP772 with SacII and ClaI, blunting the ends with T4 DNA polymerase, ligating with NotI linkers (5'-AGCGGCCGCT-3'), cutting with NotI, and ligating with a NotI fragment of pTP857  containing the tetR and tetA genes of transposon Tn10.
lac::cat PaeR7 site variants
pTP819, in which the cat gene is flanked on both sides by lac sequences, PaeR7 sites, and λ sequences (for crossing into the phage), has been described . pTP828 and pTP829 were described previously (without names) as intermediate plasmids in the construction of pTP819 ; each bears the cat gene with a single lac flank and PaeR7 site. pTP922 was constructed by deleting the lac and cat sequences between the two PaeR7 sites in pTP819. (In this and other plasmid constructions, PaeR7 sites were cut with XhoI, an isoschizomer). pTP926, pTP927, and pTP928 were constructed by ligating the oligonucleotide 5'-TCGACAGTCTAGACTG-3' into the PaeR7 sites of pTP828, pTP829, and pTP922, respectively, eliminating the PaeR7 sites and replacing them with XbaI sites. pTP929 was constructed by ligating the XbaI-NcoI fragment of pTP926 containing the N-terminal coding sequences of cat and the NcoI-XbaI fragment of pTP927 containing the C-terminal coding sequence of cat into the XbaI site of pTP928. The orientation of the reconstructed cat gene is the same as in pTP819. pTP930 was constructed by ligating together the large ApaI-SacII fragment of pTP929 and the small ApaI-SacII fragment of pTP819. pTP931 was constructed by ligating together the small ApaI-SacII fragment of pTP929 and the large ApaI-SacII fragment of pTP819.
pTP921 (short-flank lac::cat)
pTP921 was constructed by ligating together two XhoI-digested DNAs: pTP922 and a PCR product made by amplifying the cat gene from a Tn9-containing E. coli strain with primers 5'-GACGCACTCGAGGCGTTAACCGTCACGAGCATCATCCTCTGCATGGTCAGGCCGGCCACTGGAGCACCTCAAAAACACCA-3' and 5'-GACGCACTCGAGGCACACAGCGCCCAGCCAACACAGCCAAACATCCGCGCGGGCCCGACCGGGTCGAATTTGCTTTCGAA-3'. The presence of the expected sequences from the synthetic oligonucleotides in pTP921 DNA was verified by automated sequencing (data not shown).
lac flank variants
pTP988 (left lac flank only) was constructed by replacing the XbaI-ApaI lacZY segment of pTP930 with a mixture of two oligonucleotides, 5'-CTAGTTGCAAGCTTGGGCC-3' and 5'-CAAGCTTGCAA-3'. pTP989 (right lac flank only) was constructed by replacing the NgoMI-XhoI lacZ segment of pTP930 with a mixture of two oligonucleotides, 5'-CCGGCAAGCTTGCTGGTGGGCAA-3' and 5'-TCGATTGCCACCAGCAAGCTTG-3'. pTP995 (no lac flank) was constructed by ligating together the small NcoI fragment of pTP988 and the large, ori-containing NcoI fragment of pTP989.
pTP1032 was constructed by ligating together the XbaI- and PaeR7-ended cat-containing fragment of pTP930 and the XbaI- and PaeR7-ended ori-containing fragment of pTP931. pTP1033 was constructed by ligating together the XbaI- and PaeR7-ended cat-containing fragment of pTP931 and the XbaI- and PaeR7-ended ori-containing fragment of pTP930.
pTP1016 was constructed by ligating into the NotI site of pTP809  the NotI-digested PCR product made by amplifying the cat gene and flanking sequences from strain TP872 (Δ lac::cat) with primers 5'-CATCATCACGCGGCCGCGACGTTTGCCGCTTCTGAA-3' and 5'-ATCATCCACGCGGCCGCTGCGTTTTGCACCAGTACG-3'. In pTP1016, the cat gene is flanked closely by unique SphI and BsrGI sites. pTP1027 was constructed by electroporating SphI- and BsrGI-digested pTP1016 into strain TP829 (lac+); a plasmid formed by gap repair was isolated. pTP1028 was constructed by ligating SphI-digested pTP1027 with the oligonucleotide 5'-GTTGTACAACCATG-3', converting the SphI site into a BsrGI site. pTP1034 was constructed by cutting pTP1028 with BsrGI, ligating the two fragments back together, and screening for plasmids in which the lac genes were inverted relative to their flanking sequences.
R6K oriγ lac::cat plasmids
pTP1029, a tetracycline resistance bearing vector capable of replicating only in cells expressing R6K pir function, was constructed by ligating together two AatII- and Bam-digested DNA species: a PCR product made by amplifying a Tn10-containing E. coli strain with primers 5'-TCAACGTAAATGCATGGACGTCCTCGACATCTTGGTTACCGT-3' and 5'-TGTACACCATGAATTGGATCCCGCGGAATAACATCATTTGG-3'; and the ori-containing fragment of plasmid pLD54 . pTP1018, 1019, 1020, 1044, and 1045 were constructed by ligating the BamHI lac::cat-containing fragments of pTP819, 930, 931, 1032, and 1033, respectively, into the BamHI site of pTP1029.
Rearranged lac::cat variants (Table 3)
pTP1047 was constructed by ligating the complementary oligonucleotides 5'-TCGTCTAGAGT-3' and 5'-CCGGACTCTAGACGAAGCT-3' between the SacI and NgoMIV sites of pTP1019. pTP1051 was constructed by ligating the complementary oligonucleotides 5'-CAGCATGCAT-3' and 5'-CTAGATGCATGCTGGTAC-3' between the KpnI and XbaI sites of pTP930. pTP1052 was constructed by ligating the complementary oligonucleotides 5'-CAGCATGCAGGGCC-3' and 5'-CTGCATGCTGGTAC-3' between the KpnI and ApaI sites of pTP1019. pTP1053 was constructed by ligating the complementary oligonucleotides 5'-GGCATGCAGGTTCTTTGAGTCCTTTGGGCGGCCGCGGGCC-3' and 5'-CGCGGCCGCCCAAAGGACTCAAAGAACCTGCATGCCGC-3' between the SacII and ApaI sites of pTP1019. pTP1054 was constructed by ligating the complementary oligonucleotides 5'-CCGGCGCGGCCGCAGGTTCTTTGAGTCCTTTGGTGTACAGAGCT-3' and 5'-CTGTACACCAAAGGACTCAAAGAACCTGCGGCCGCG-3' between the NgoMIV and SacI sites of pTP1020. pTP1055 was constructed by ligating the small SphI-NotI fragment of pTP1016 between the SphI and NotI sites of pTP1053. pTP1056 was constructed by ligating the small NotI-BsrGI fragment of pTP1016 between the NotI and BsrGI sites of pTP1054. pTP1057 was constructed by ligating the BamHI cat-containing fragment of pTP1051 into the BamHI site of pTP1029.
Δ recBCD::Ptac-gam-bet-exo-pae-cI variants
pTP822, which bears a synthetic Ptac-gam-bet-exo-pae-cI operon flanked by sequences upstream from recC on one side and sequences internal to recD on the other, has been described . pTP978 was constructed by ligating together two NcoI- and XbaI-digested DNA species: pTP822, and a PCR product made by amplifying the cI gene from E. coli strain TP507 with the same primers used in the construction of pTPP822. This construction has the effect of simply deleting the pae genes from pTP822. pTP996 was constructed by deleting the C-terminal coding sequences of bet and the N-terminal coding sequences of exo between the two HpaI sites of pTP978. pTP979 was constructed by ligating together two NcoI- and XbaI-digested DNAs: pTP822 and a PCR product made by amplifying the bla gene from pBR322 with primers 5'-CCACCAATCATCCATGGCGCGGAACCCCTATTTGTTT-3' and 5'-TTGTTGGACGATCTAGAGGTCTGACAGTTACCAATGC-3'. This construction has the effect of replacing pae and cI with bla.
λ lac::cat819 and λ lac::cat819 nin5 have been described . λΔ nin::tet859 was made by crossing λ wild type with plasmid pTP859, infecting a tetracycline-sensitive strain with the resulting lysate, and selecting a tetracycline-resistant lysogen. The Δ nin::tet859 substitution replaces λ bp 40388–43825 with the tetR and tetA genes of transposon Tn 10.
The lac::cat921, 929, 930, 931, 988, 989, 995, 1032, and 1033 substitutions were crossed into λ wild type and/or λ Δ nin::tet859. The parent phages were crossed with the cat substitution-bearing plasmids. Phages which had acquired the substitution were either selected by plating on a strain lysogenic for phage P2 (Spi- phenotype), or identified by their clear-plaque morphologies (all the cat substitutions replace the cIII gene). The structures of the substituted phages were all verified by their ability to generate specific products when used as templates in PCR (not shown).
Phages were crossed with plasmids by spotting enough phage to make a confluent zone of lysis on a lawn of a sensitive bacterial strain bearing the parent plasmid. After overnight incubation at 37°C, material from the zone was collected in TM (10 mM Tris-HCl pH 7.5, 10 mM MgSO4), and shaken with chloroform. Two methods for crosses monitoring recombination between phage-injected DNA and the bacterial chromosome, both previously described, were employed: high-multiplicity infection of log phase cultures , and low-multiplicity infection of cells grown to stationary phase by standing overnight incubation .
Crosses involving recombination between bacterial chromosomes and electroporated DNA fragments were carried out as described by Murphy and Campellone . The DNA fragments were generated by digesting various plasmids with restriction endonucleases. Amounts of DNA used varied among experiments, corresponding to 250–500 ng of the 3595-bp lac::cat Xho fragment; within an experiment, equimolar amounts of different recombining linear species were used. DNA was quantitated by running samples in an agarose gel, staining with ethidium bromide, and measuring fluorescence of the bands by the use of a Kodak Gel-Logic 200 system with 1-D software.
The structures of various Red-generated duplications in the E. coli were verified by PCR. Colonies were picked and added to 40 μl mixtures containing 1 unit Taq polymerase, 63 μM dNTPs, 1.88 mM MgCl2, 5% (v/v) dimethylsulfoxide, 20 mM Tris-HCl pH 8.4, 50 mM KCl, and primers at 0.7 μM. Samples were heated to 95C for 5 min, then put through 30 cycles of 94C for 1 min, 55C for 1 min, 72C for 2–4 min, depending upon the length of the expected products. Primers for demonstrating the type I, II, and III tfa junctions were combined in a single mixture of 4 oligonucleotides: Jsp1 (GTTGAATGGGCGGATGCTAA), Jsp2 (TCTTCCACCAGAAAGCTACC), JncA (TGCCGTGTGAACGGTTTAC), and Jnc2 (TTCTAGCCCCATCATCTGTG). Primers for spanning the ACF-generated duplication (see Figure 7) were Ddi1 (CTCTTTCCGTTACGGGACAC) and Ddi2 (TGGTGAACATGATGCCGACA). Primers for spanning the GCB-generated duplication were Edi1 (CGCCGAAATCCCGAATCTCT) and Edi2 (ACCGGCATACTCTGCGACAT). Primers for demonstrating the DLP12 cos junction were Csj1 (GATTGAGCGTGAAGTCTGTTTGTG) and Csj2 (CGAATAGTCGGCTCAACGTGGGTT).
polymerase chain reaction. X-Gal: 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside. IPTG: isopropyl β-D-thiogalactopyranoside. bp: base pairs.
Yanxia Guo and Daniel Aiello provided excellent technical assistance. ARP thanks Kenan Murphy for helpful advice and discussions. This research was supported by grant MCB-0234991 from the National Science Foundation and grant GM51609 from the National Institutes of Health.
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