The p68 and p72 DEAD box RNA helicases interact with HDAC1 and repress transcription in a promoter-specific manner
© Wilson et al; licensee BioMed Central Ltd. 2004
Received: 11 March 2004
Accepted: 06 August 2004
Published: 06 August 2004
p68 (Ddx5) and p72 (Ddx17) are highly related members of the DEAD box family and are established RNA helicases. They have been implicated in growth regulation and have been shown to be involved in both pre-mRNA and pre-rRNA processing. More recently, however, these proteins have been reported to act as transcriptional co-activators for estrogen-receptor alpha (ERα). Furthermore these proteins were shown to interact with co-activators p300/CBP and the RNA polymerase II holoenzyme. Taken together these reports suggest a role for p68 and p72 in transcriptional activation.
In this report we show that p68 and p72 can, in some contexts, act as transcriptional repressors. Targeting of p68 or p72 to constitutive promoters leads to repression of transcription; this repression is promoter-specific. Moreover both p68 and p72 associate with histone deacetylase 1 (HDAC1), a well-established transcriptional repression protein.
It is therefore clear that p68 and p72 are important transcriptional regulators, functioning as co-activators and/or co-repressors depending on the context of the promoter and the transcriptional complex in which they exist.
The DEAD/H box family of RNA helicases has been demonstrated to be involved in virtually all processes that require manipulation of RNA including transcription, pre-mRNA and pre-rRNA processing, RNA export, ribosome assembly and translation . Although, in vitro, several members of this family have been shown to unwind RNA duplexes, relatively few appear to be true processive helicases and it is clear that, in the cell, many are likely to be involved in unwinding of short base paired regions of RNA or in the modulation of RNA-protein interactions.
DNA helicases belong to a superfamily of proteins that is distantly related to DEAD/H box RNA helicases and includes the Werner syndrome protein (WRN)  and the Xeroderma pigmentosum XPB and XPD proteins , which have well established roles in transcription. Although the functions of DEAD/H box RNA helicases in other cellular processes, such as pre-mRNA processing and translation have been well studied, their role in transcriptional regulation is only now emerging. Examples of DEAD/H box RNA helicases involved in transcription include RNA helicase II (RHII/Gu) and RNA helicase A (RHA/NDHII). RHII/Gu was demonstrated to be a cofactor for c-Jun-activated transcription  and was shown to translocate from the nucleolus to the nucleoplasm after UV or anisomycin treatment (which activates JNK signalling). Although RHII/Gu was found to associate with phosphorylated c-Jun in a non-phosphorylated state, this association was observed to increase after anisomycin treatment, implying a stronger interaction when c-Jun is phosphorylated . RHA is a homologue of the Drosophila maleless (MLE) gene product  and is thought to be important for gene dosage compensation on the X-chromosome . RHA has been shown to be required for complex formation between the transcriptional co-activator, CREB binding protein (CBP), and RNA polymerase II . Furthermore, different regions of the RNA helicase protein were found to interact with both CBP and RNA polymerase II. The association of RHA with RNA polymerase II was further investigated, and narrowed down to a 50 amino acid stretch, outwith the conserved helicase motifs ; this study also showed that RHA could regulate CREB-dependent transcription either through recruitment of Pol II or by ATP-dependent mechanisms. A later study reported that RHA acts as a bridging molecule between the breast tumour specific transcriptional activator, BRCA1 and the RNA polymerase II holoenzyme complex . These reports thus provide clear evidence of a role for RNA helicases as transcription factors.
p68 is a prototypic member of the DEAD box family of proteins  and an established RNA helicase . The subsequent discovery of p72  and the finding that p68 and p72 share remarkable homology (90% over the central conserved core and 60% and 30% at the N- and C-terminal extensions respectively) suggests that these proteins may form a specific sub-group of DEAD box proteins and may have similar, but perhaps subtly different, functions in the cell, perhaps through interaction with different RNA substrates or proteins. In vitro, both proteins exhibit the RNA-dependent ATPase and RNA helicase activities characteristic of members of the DEAD box family [10–14] and have also been reported to catalyse rearrangement of RNA structure via branch migration . Moreover p68 and p72 can interact with each other, as well as self-associate, and appear to preferentially form heterodimers in cells . This provides the potential for a wide range of functions for p68 and p72 with the possibility of their co-operation in some contexts.
More recently p68 and p72 have been shown to be involved in a range of processes in the cell, including pre-mRNA and pre-rRNA processing and alternative splicing [16–18]. p68 and p72 have also been shown to be growth- and developmentally-regulated [19–22] and, furthermore, p68 appears to be over-expressed and poly-ubiquitylated in colorectal tumours . Interestingly p68 has been shown to act as a transcriptional co-activator, specific for the activation function 1 (AF-1) domain of estrogen receptor alpha (ERα) . This interaction was dependent upon phosphorylation of AF-1 at serine118, a residue phosphorylated by mitogen-activated protein kinase (MAPK). Interestingly the RNA helicase function of p68 appeared to be dispensable for this activity as a mutant p68 (Lys144 to Arg) in the ATP binding site (conserved motif I) retained the ability to co-activate ERα, although in a later study RNA binding appeared to be required . p72 was subsequently shown to share this property and both proteins were shown to interact with the activation domain 2 (AD2) of p160 co-activators . Furthermore p68 was also found to interact with the CBP co-activator and RNA polymerase II . More recently p68 has been shown to be recruited to the promoter of the ERα target gene pS2 , suggesting a direct involvement in transcriptional regulation.
In this report we explore further potential mechanisms through which p68 and p72 may contribute to transcriptional regulation. We find that, in some contexts, p68 and p72 can also act as transcriptional repressors and that these proteins exhibit clear promoter specificity in this function. By directing GAL4-tagged p68/p72 (GAL4 DNA binding domain aa1-147) to promoters containing GAL4 binding sites, we show that both p68 and p72 can repress transcription from the herpes virus thymidine kinase (TK) promoter but not the simian virus 40 promoter/enhancer. Moreover, while p72 can repress transcription from the Adenovirus major late promoter, p68 appears to have no effect, suggesting that these proteins do not behave in an identical way in all contexts. Furthermore we show, by co-immunoprecipitation, that both p68 and p72 interact with histone deacetylase 1 (HDAC1) and that HDAC1/p68/p72 co-elute by gel filtration, indicating that they exist in the same complex in the cell and suggesting a possible mechanism by which these proteins may exert their repressive effect.
p68 and p72 differentially repress constitutively active promoters/enhancers
The repression of transcription is an active process
To confirm that the repressive effect observed is not due to an artefact of the assay conditions we initially repeated the experiment with the TK-CAT reporter plasmid, using non-tagged p68 or p72 or the pcDNA3 expression plasmid vector alone (Figure 2a). In this experiment neither p68 nor p72 significantly reduce transcription of the TK-CAT reporter, suggesting that the repression observed with the GAL4-tagged p68/p72 plasmids (Figure 1) is not due to competing out of an essential factor required for TK-CAT transcription but, instead, implies an active mechanism of repression in which the p68/p72 proteins are required to be directed to the TK-CAT promoter via the GAL4 tag.
The possibility still remained that p68 and p72 were repressing transcription by direct interference with the transcriptional machinery of the TK-CAT promoter (e.g. perhaps by physically blocking the promoter). To rule out this possibility, we used a similar TK-CAT promoter construct, which had, however, a 1.6 kb DNA 'spacer' between the GAL4 binding sites and the promoter (the inserted DNA is reported to have no effect on transcription ). Both GAL4-p68 and -p72 retained the ability to repress transcription, almost to the same degree as previously (Figure 2b). Furthermore, a titration using the GAL4- p68/p72 fusion proteins clearly shows a dose-dependent concentration curve (Figure 2c) as would be expected for active repression. In addition, p68 and p72 have been observed to interact with p300/CBP co-activators [24, 26]. Therefore it remained possible that the observed transcriptional repression was due to competition for, or interference with, p300/CBP. If this were the case co-expression of p300/CBP would be expected to relieve repression by p68/p72. As shown in Figure 2d, no such relief of expression was observed. Thus our findings that transcriptional repression by p68/p72 was dose-dependent and not due to steric blocking of the promoter or competition/interference with p300/CBP, suggest that it is an active process.
Deletion experiments do not identify a distinct repression domain for p68 or p72 but reveal an activation domain
p72 immunoprecipitates a HDAC activity
To examine further the involvement of HDACs in transcriptional repression by p68/p72 we determined whether p68 and/or p72 co-immunoprecipitate a HDAC activity in cells. Plasmids expressing myc-tagged p68 and p72 were expressed in 293 cells (with myc tag plasmid vector as a negative control) and the tagged proteins were immunoprecipitated from cell lysates using the myc epitope antibody, 9E10. Equal amounts of immunoprecipitated protein bound to the antibody, as confirmed by western blotting (Figure 4b), were then used in an HDAC activity assay (Biomol) in the presence and absence of TSA. In this assay (Figure 4c) p72 was found to co-immunoprecipitate a HDAC activity, which is abolished by TSA, while p68 did not. These findings are consistent with p72 interacting with a HDAC and repressing transcription in a HDAC-dependent manner.
p68 and p72 associate with HDAC1 in cells
We have shown that the highly related DEAD box RNA helicases p68 and p72 act as repressors of transcription in a promoter-context manner. When targeted to the TK-CAT promoter-reporter construct they both strongly repress transcription (Figure 1). Furthermore, this transcriptional repression does not appear to be due to either squelching or physical blocking of the transcription apparatus (Figure 2), implying an active transcriptional mechanism. Moreover repression of TK-CAT was observed in several cell lines (U2OS, 293, MCF-7) suggesting that it is not cell line dependent. In order to determine whether this repression activity exhibited any promoter specificity we also tested the ability of p68 and p72 to repress transcription of other constitutively active promoter-reporter constructs with high basal levels of transcription, namely MLP-CAT and SV40-CAT. Interestingly MLP-CAT revealed a difference in the ability of p68 and p72 to repress transcription, with p72 strongly repressing transcription of this promoter-reporter and p68 having no effect (Figure 1). This observation suggests that, although highly homologous (70% overall identity at the amino acid level ) p68 and p72 proteins may act differently in some contexts, perhaps through the association with different protein partners. Neither p68 nor p72 repressed transcription of SV40-CAT (Figure 1) suggesting that the repression by p68 and p72 is promoter context-dependent, an observation that has been reported for other transcription factors . These findings thus are consistent with the observed repression activity of p68/p72 being an active process. Interestingly, in this context, another DEAD box protein DP103 (Ddx20) has been found to act as a co-repressor of the Ets repressor METS/PE1 .
Using a series of deletion derivatives of p68 and p72 we identified three domains, within the core conserved among the DEAD box family of proteins, which can independently repress transcription (Figure 3c). Moreover regions encompassing residues 1–478 of p68 and 1–474 of p72, which contain the complete conserved core (Figure 3c), can repress transcription as well as the respective full-length proteins (Figure 3a,3b). In contrast, the C-terminal extension of both proteins acts as a transcriptional activator in this context (Figure 3a,5) consistent with earlier reports of these proteins acting as transcriptional co-activators [24, 25]. Thus, using this system, we have shown that there are separable transcriptional repression and activation domains within p68 and p72.
Since HDAC proteins have been extensively implicated in the repression of transcription, it was important to examine whether these proteins are likely to play a role in transcriptional repression by p68 and/or p72. Firstly, the ability of the HDAC inhibitor, TSA, to relieve repression was examined. No effect was observed on repression of TK-CAT (data not shown) implying the involvement of a HDAC-independent mechanism in repression of the TK promoter. In contrast, repression of MLP-CAT by p72 was relieved two-fold compared with the vector control (Figure 4a) suggesting the involvement of HDACs in this process. (Since p68 did not repress MLP-CAT transcription, the lack of effect by TSA is not surprising.) Supporting these data, p72 was found to co-immunoprecipitate a HDAC activity which was abolished by the addition of TSA, while p68 did not (Figure 4b). We chose to investigate whether p68 and/or p72 associate with HDAC1, since it is a well-studied example of Class I HDACs. Both p68 and p72 co-immunoprecipitate with HDAC1 (Figures 6 and 7); furthermore HDAC1, p68 and p72 co-elute in similar sized complexes by gel-filtration, which are of an appropriate size (Figure 5) supporting the idea of interactions between p68/p72 and HDAC1 in cells. Moreover, the finding that these proteins co-immunoprecipitate and co-elute from extracts which had been treated with DNase/RNase suggest that these represent protein-protein interactions rather than merely interactions via nucleic acid. While an interaction between HDAC1 and p68 is not supported by the results of the HDAC assay, or the TSA experiment, it is possible that, in some instances, p68 does recruit HDAC1 and that this mechanism is not being triggered in the MLP-CAT assay or HDAC assay. Alternatively, it is possible that the observed co-immunoprecipitation of p68 and HDAC1 is occurring through the interaction between p68 and p72  or that HDAC1 associated with p68 may have other, possibly non-transcriptional, roles [35, 36]. However, the data are entirely consistent with p72 recruiting HDAC1 to achieve active repression of transcription. Future investigations should also reveal whether the differential ability of p68 and p72 to recruit active HDAC proteins is responsible for the difference observed upon the MLP promoter.
Our attempts at correlating the different repressive functions of p68 and p72 to specific domains of the respective proteins, using deletion derivatives, were unsuccessful, as the equivalent regions of both either caused transcriptional repression or activation. While this might suggest that both helicases repress transcription in the same manner, it is more likely that the HDAC recruitment by p72 may be an additional mechanism of repression, used at specific promoters. We also cannot rule out the recruitment of other repression complexes at this stage. Our findings that p68 and p72 differ in their ability to repress the MLP promoter and to recruit HDAC activity suggest that, at least in some contexts, p68 and p72 repress transcription by different mechanisms.
In summary, it is clear that p68 and p72 act to repress transcription in a differential manner dependent upon promoter context. It will be important to determine which endogenous promoters are subject to repression by p68/p72 in a physiological context. However, until the signal transduction pathways, which target these proteins to the appropriate promoters, are elucidated it will be necessary to use a targeting system (such as GAL4) to undertake a molecular analysis of transcriptional repression by p68 and p72. Since it is now clear that p68/p72 can act both to activate and repress transcription future work will involve dissection of the transcriptional activation/repression complexes in which p68 and p72 are involved, as well as characterisation of the molecular 'switch' which determines whether these proteins will be part of transcriptional activation or repression complexes.
We have shown that the highly related RNA helicases p68 and p72 can repress transcription in a promoter context-dependent manner. Both proteins associate with HDAC1, a well-established transcriptional repressor protein. Strikingly, however, p68 and p72 behave differently in their ability to repress transcription from different promoters and in their ability to recruit HDAC activity suggesting that they may, at least in some contexts, repress transcription by different mechanisms.
U2OS human osteosarcoma cells and 293 human embryo kidney cells were maintained at 5% CO2 at 37°C in DMEM with 10% FBS, 2 mM L-glutamine, 100 units/ml penicillin and 100 μg/ml streptomycin (all supplied by Invitrogen).
A pcDNA3-GAL4 expression plasmid was used to express full-length and deletion derivatives of p68/p72/p82 tagged at the N-terminus with the DNA binding domain (amino acids 1–147) of GAL4 . The majority of deletion derivatives were created by PCR and inserted, as Bam HI/Eco RI fragments, in frame with the GAL4 tag. The CAT reporter constructs have been described previously: TK-CAT , TK-Spacer-CAT , MLP-CAT and SV40-CAT . The MLP-CAT and SV40-CAT plasmids were a kind gift from Douglas Dean (Washington University, St Louis, USA), and the TK-spacer-CAT plasmid was kindly provided by Dr. Alain Nepveu (McGill University, Canada). Untagged p300, CBP, p68, p72 were expressed from pcDNA3. A myc-tagged derivative of pSG5 (Stratagene)  was used to express the myc-tagged p68/p72 or the myc epitope alone as negative control.
p68: The antibodies used were the mouse monoclonal antibody PAb 204 and the rabbit polyclonal antibody 2906, generated against the C-terminal 15 amino acids of p68 . PAb 204 was originally generated against the SV40 large T antigen but it cross-reacts with p68 . It is specific for p68 in cells that are not infected or transformed by SV40. p72: A rabbit anti-peptide polyclonal antibody generated against amino acids 624 to 638  was used to detect p72/p82 in fractions from gel filtration. Myc epitope: A mouse monoclonal antibody (9E10) was used to detect proteins tagged with the myc epitope (MRQKLISEEDL). HDAC1: A rabbit polyclonal antibody (Oncogene Research Products) was used both for immunoprecipitation and western blotting. GAL4- a mouse monoclonal antibody (Santa Cruz) was used to detect GAL4-tagged proteins. A negative control rabbit IgG antibody for immunoprecipitation was obtained from R&D systems and appropriate anti-mouse and anti-rabbit secondary antibodies were obtained from DAKO.
Transient transfections and chloramphenicol acetyl-transferase (CAT) assays
3×105 U2OS cells were seeded for transfections, which were performed as previously described . Cells were co-transfected with the appropriate CAT reporter construct and GAL4-tagged p68/p72, and in each case the DNA was made up to a total of 10 μg with Bluescript DNA (Stratagene). CAT activity was determined 48 hr after transfection using 100 μg of total protein from cleared whole cell lysates. Typically each experiment was performed in triplicate within each assay, and each assay was repeated 3 to 5 times. Where applicable, the HDAC inhibitor Trichostatin A (TSA) (Upstate Biotechnology) was added, at a final concentration of 300 nM, 16 hr post transfection.
Nuclear extract preparation and co-immunoprecipitation
Nuclear extracts were prepared from U2OS and 293 cells essentially as described in  except that the NaCl concentration was reduced to 330 mM NaCl, then diluted to 150 mM NaCl (after nuclear lysis) and treated with RNAse/DNAse. The extract was pre-cleared in buffer D [20 mM Hepes (pH7.9), 150 mM NaCl, 0.5 mM DTT, 20% (v/v) glycerol, 10 mM NaF and protease inhibitor cocktail (Roche)] with protein G sepharose beads for 30 min. at 4°C. Immunoprecipitations were carried out in the presence of appropriate antibodies and protein G sepharose beads for one hour at 4°C. After washing in buffer D plus 0.1% Igepal (Sigma), immunoprecipitated proteins were separated by SDS-PAGE and western blotted using standard conditions and appropriate primary and secondary antibodies. Immunoreactive proteins were detected using the ECL method (Amersham).
293 cell lysates were prepared in RIPA buffer [50 mM Tris-HCl (pH8.0), 150 mM NaCl, 1% Igepal, 0.1% SDS, 1% Na deoxycholate, 1 mM EDTA and protease inhibitor cocktail (Roche)]. After treatment with RNase/DNase, lysates were fractionated on a Pharmacia Superose 6 HR column in 50 mM Tris-HCl (pH7.5), 150 mM NaCl, 10% glycerol and 1 mM benzamidine, using a Pharmacia AKTA FPLC system. 0.5 ml fractions were collected and alternate fractions were analysed by western blotting. Molecular weight standards from Pharmacia were used to calibrate the column.
293 cells were transfected with either pSG5-myc vector, pSG5-p68-myc, or pSG5-p72-myc. Cells were lysed in buffer B 48 hr after transfection. The lysate was then diluted in buffer A and myc-tagged proteins immunoprecipitated with 9E10 (myc epitope) antibody as described above. The HDAC assay employs Fleur de lys substrate, which contains an acetylated lysine side chain (Biomol) and was performed according to manufacturers instructions. Antibody-bound beads were washed in HDAC assay buffer prior to being added to the 96-well plate, to remove immunoprecipitation buffer. Reactions were incubated for 30 min at 37°C with or without the addition of 1 μM TSA. Samples were excited at 360 nm and emitted at 460 nm and were read in a fluorometer. Typically each assay was performed 3 times.
We thank David Lane and David Meek for helpful discussions. This work was supported by Tenovus Scotland and the BBSRC (studentship to BJW).
- Tanner NK, Linder P: DExD/H box RNA helicases: from generic motors to specific dissociation functions. Mol Cell. 2001, 8: 251-262. 10.1016/S1097-2765(01)00329-XView ArticlePubMed
- Balajee AS, Machwe A, May A, Gray MD, Oshima J, Martin GM, Nehlin JO, Brosh R, Orren DK, Bohr VA: The Werner syndrome protein is involved in RNA polymerase II transcription. Mol Biol Cell. 1999, 10: 2655-2668.PubMed CentralView ArticlePubMed
- Tirode F, Busso D, Coin F, Egly JM: Reconstitution of the transcription factor TFIIH: assignment of functions for the three enzymatic subunits, XPB, XPD, and cdk7. Mol Cell. 1999, 3: 87-95. 10.1016/S1097-2765(00)80177-XView ArticlePubMed
- Westermarck J, Weiss C, Saffrich R, Kast J, Musti AM, Wessely M, Ansorge W, Seraphin B, Wilm M, Valdez BC, Bohmann D: The DEXD/H-box RNA helicase RHII/Gu is a co-factor for c-Jun-activated transcription. Embo J. 2002, 21: 451-460. 10.1093/emboj/21.3.451PubMed CentralView ArticlePubMed
- Lee CG, Hurwitz J: Human RNA helicase A is homologous to the maleless protein of Drosophila. J Biol Chem. 1993, 268: 16822-16830.PubMed
- Kuroda MI, Kernan MJ, Kreber R, Ganetzky B, Baker BS: The maleless protein associates with the X chromosome to regulate dosage compensation in Drosophila. Cell. 1991, 66: 935-947. 10.1016/0092-8674(91)90439-6View ArticlePubMed
- Nakajima T, Uchida C, Anderson SF, Lee CG, Hurwitz J, Parvin JD, Montminy M: RNA helicase A mediates association of CBP with RNA polymerase II. Cell. 1997, 90: 1107-1112. 10.1016/S0092-8674(00)80376-1View ArticlePubMed
- Anderson SF, Schlegel BP, Nakajima T, Wolpin ES, Parvin JD: BRCA1 protein is linked to the RNA polymerase II holoenzyme complex via RNA helicase A. Nat Genet. 1998, 19: 254-256. 10.1038/930View ArticlePubMed
- Ford MJ, Anton IA, Lane DP: Nuclear protein with sequence homology to translation initiation factor eIF-4A. Nature. 1988, 332: 736-738. 10.1038/332736a0View ArticlePubMed
- Hirling H, Scheffner M, Restle T, Stahl H: RNA helicase activity associated with the human p68 protein. Nature. 1989, 339: 562-564. 10.1038/339562a0View ArticlePubMed
- Lamm GM, Nicol SM, Fuller Pace FV, Lamond AI: p72: a human nuclear DEAD box protein highly related to p68. Nucleic Acids Res. 1996, 24: 3739-47 . 10.1093/nar/24.19.3739PubMed CentralView ArticlePubMed
- Iggo RD, Lane DP: Nuclear protein p68 is an RNA-dependent ATPase. Embo J. 1989, 8: 1827-1831.PubMed CentralPubMed
- Rossler OG, Straka A, Stahl H: Rearrangement of structured RNA via branch migration structures catalysed by the highly related DEAD-box proteins p68 and p72. Nucleic Acids Res. 2001, 29: 2088-2096. 10.1093/nar/29.10.2088PubMed CentralView ArticlePubMed
- Huang Y, Liu ZR: The ATPase, RNA unwinding, and RNA binding activities of recombinant p68 RNA helicase. J Biol Chem. 2002, 277: 12810-12815. 10.1074/jbc.M200182200View ArticlePubMed
- Ogilvie VC, Wilson BJ, Nicol SM, Morrice NA, Saunders LR, Barber GN, Fuller-Pace FV: The highly related DEAD box RNA helicases p68 and p72 exist as heterodimers in cells. Nucleic Acids Res. 2003, 31: 1470-1480. 10.1093/nar/gkg236PubMed CentralView ArticlePubMed
- Bond AT, Mangus DA, He F, Jacobson A: Absence of Dbp2p alters both nonsense-mediated mRNA decay and rRNA processing. Mol Cell Biol. 2001, 21: 7366-7379. 10.1128/MCB.21.21.7366-7379.2001PubMed CentralView ArticlePubMed
- Lee CG: RH70, a Bidirectional RNA Helicase, Co-purifies with U1snRNP. J Biol Chem. 2002, 277: 39679-39683. 10.1074/jbc.C200337200View ArticlePubMed
- Honig A, Auboeuf D, Parker MM, O'Malley BW, Berget SM: Regulation of Alternative Splicing by the ATP-Dependent DEAD-Box RNA Helicase p72. Mol Cell Biol. 2002, 22: 5698-5707. 10.1128/MCB.22.16.5698-5707.2002PubMed CentralView ArticlePubMed
- Stevenson RJ, Hamilton SJ, MacCallum DE, Hall PA, Fuller Pace FV: Expression of the 'dead box' RNA helicase p68 is developmentally and growth regulated and correlates with organ differentiation/maturation in the fetus. J Pathol. 1998, 184: 351-9 Issn: 0022-3417. 10.1002/(SICI)1096-9896(199804)184:4<351::AID-PATH1235>3.0.CO;2-CView ArticlePubMed
- Ip FC, Chung SS, Fu WY, Ip NY: Developmental and tissue-specific expression of DEAD box protein p72. Neuroreport. 2000, 11: 457-462.View ArticlePubMed
- Seufert DW, Kos R, Erickson CA, Swalla BJ: p68, a DEAD-box RNA helicase, is expressed in chordate embryo neural and mesodermal tissues. J Exp Zool. 2000, 288: 193-204. 10.1002/1097-010X(20001015)288:3<193::AID-JEZ1>3.0.CO;2-VView ArticlePubMed
- Kitamura A, Nishizuka M, Tominaga K, Tsuchiya T, Nishihara T, Imagawa M: Expression of p68 RNA helicase is closely related to the early stage of adipocyte differentiation of mouse 3T3-L1 cells. Biochem Biophys Res Commun. 2001, 287: 435-439. 10.1006/bbrc.2001.5577View ArticlePubMed
- Causevic M, Hislop RG, Kernohan NM, Carey FA, Kay RA, Steele RJ, Fuller-Pace FV: Overexpression and poly-ubiquitylation of the DEAD-box RNA helicase p68 in colorectal tumours. Oncogene. 2001, 20: 7734-7743. 10.1038/sj.onc.1204976View ArticlePubMed
- Endoh H, Maruyama K, Masuhiro Y, Kobayashi Y, Goto M, Tai H, Yanagisawa J, Metzger D, Hashimoto S, Kato S: Purification and identification of p68 RNA helicase acting as a transcriptional coactivator specific for the activation function 1 of human estrogen receptor alpha. Mol Cell Biol. 1999, 19: 5363-5372.PubMed CentralView ArticlePubMed
- Watanabe M, Yanagisawa J, Kitagawa H, Takeyama K, Ogawa S, Arao Y, Suzawa M, Kobayashi Y, Yano T, Yoshikawa H, Masuhiro Y, Kato S: A subfamily of RNA-binding DEAD-box proteins acts as an estrogen receptor alpha coactivator through the N-terminal activation domain (AF- 1) with an RNA coactivator, SRA. Embo J. 2001, 20: 1341-1352. 10.1093/emboj/20.6.1341PubMed CentralView ArticlePubMed
- Rossow KL, Janknecht R: Synergism between p68 RNA helicase and the transcriptional coactivators CBP and p300. Oncogene. 2003, 22: 151-156. 10.1038/sj.onc.1206067View ArticlePubMed
- Metivier R, Penot G, Hubner MR, Reid G, Brand H, Kos M, Gannon F: Estrogen receptor-alpha directs ordered, cyclical, and combinatorial recruitment of cofactors on a natural target promoter. Cell. 2003, 115: 751-763. 10.1016/S0092-8674(03)00934-6View ArticlePubMed
- Gregory DJ, Garcia-Wilson E, Poole JC, Snowden AW, Roninson IB, Perkins ND: Induction of transcription through the p300 CRD1 motif by p21WAF1/CIP1 is core promoter specific and cyclin dependent kinase independent. Cell Cycle. 2002, 1: 343-350.View ArticlePubMed
- Mailly F, Berube G, Harada R, Mao PL, Phillips S, Nepveu A: The human cut homeodomain protein can repress gene expression by two distinct mechanisms: active repression and competition for binding site occupancy. Mol Cell Biol. 1996, 16: 5346-5357.PubMed CentralView ArticlePubMed
- Uhlmann-Schiffler H, Rossler OG, Stahl H: The mRNA of DEAD box protein p72 is alternatively translated into an 82- kDa RNA helicase. J Biol Chem. 2002, 277: 1066-1075. 10.1074/jbc.M107535200View ArticlePubMed
- de Ruijter AJ, van Gennip AH, Caron HN, Kemp S, van Kuilenburg AB: Histone deacetylases (HDACs): characterization of the classical HDAC family. Biochem J. 2003, 370: 737-749. 10.1042/BJ20021321PubMed CentralView ArticlePubMed
- Zhang HS, Gavin M, Dahiya A, Postigo AA, Ma D, Luo RX, Harbour JW, Dean DC: Exit from G1 and S phase of the cell cycle is regulated by repressor complexes containing HDAC-Rb-hSWI/SNF and Rb-hSWI/SNF. Cell. 2000, 101: 79-89. 10.1016/S0092-8674(00)80625-XView ArticlePubMed
- Luo RX, Postigo AA, Dean DC: Rb interacts with histone deacetylase to repress transcription. Cell. 1998, 92: 463-473. 10.1016/S0092-8674(00)80940-XView ArticlePubMed
- Klappacher GW, Lunyak VV, Sykes DB, Sawka-Verhelle D, Sage J, Brard G, Ngo SD, Gangadharan D, Jacks T, Kamps MP, Rose DW, Rosenfeld MG, Glass CK: An induced Ets repressor complex regulates growth arrest during terminal macrophage differentiation. Cell. 2002, 109 (2): 169-180. 10.1016/S0092-8674(02)00714-6View ArticlePubMed
- Matsuyama A, Shimazu T, Sumida Y, Saito A, Yoshimatsu Y, Seigneurin-Berny D, Osada H, Komatsu Y, Nishino N, Khochbin S, Horinouchi S, Yoshida M: In vivo destabilization of dynamic microtubules by HDAC6-mediated deacetylation. Embo J. 2002, 21: 6820-6831. 10.1093/emboj/cdf682PubMed CentralView ArticlePubMed
- North BJ, Marshall BL, Borra MT, Denu JM, Verdin E: The Human Sir2 Ortholog, SIRT2, Is an NAD(+)-Dependent Tubulin Deacetylase. Mol Cell. 2003, 11: 437-444. 10.1016/S1097-2765(03)00038-8View ArticlePubMed
- Chapman NR, Perkins ND: Inhibition of the RelA(p65) NF-kappaB subunit by Egr-1. J Biol Chem. 2000, 275: 4719-4725. 10.1074/jbc.275.7.4719View ArticlePubMed
- Webster GA, Perkins ND: Transcriptional cross talk between NF-kappaB and p53. Mol Cell Biol. 1999, 19: 3485-3495.PubMed CentralView ArticlePubMed
- Ayer DE, Laherty CD, Lawrence QA, Armstrong AP, Eisenman RN: Mad proteins contain a dominant transcription repression domain. Mol Cell Biol. 1996, 16: 5772-5781.PubMed CentralView ArticlePubMed
- Dignam JD, Lebovitz RM, Roeder RG: Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei. Nucleic Acids Res. 1983, 11: 1475-1489.PubMed CentralView ArticlePubMed
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